Todarodes pacificus is a commercially important squid species; however, there is little information available regarding the population genetics of this species and effective molecular markers are still unavailable. In the present study, 107 novel single nucleotide polymorphism (SNP) markers were developed using Genotyping-by-Sequencing (GBS). The observed and expected heterozygosities ranged from 0.0345 to 0.5862 and 0.0339–0.4994, respectively. The polymorphism information content ranged from 0.0333 to 0.3747. Among these SNPs, all loci have been confirmed to fit the Hardy–Weinberg equilibrium (p > 0.05). The novel SNPs developed in this study will be helpful for the genetic conservation and scientific management of Todarodes pacificus.

Todarodes pacificus是一种具有重要商业价值的鱿鱼品种；然而，关于该物种的种群遗传学信息很少，并且仍然无法获得有效的分子标记。在本研究中，使用基因分型测序 (GBS) 开发了 107 个新的单核苷酸多态性 (SNP) 标记。观察到的和预期的杂合度分别在 0.0345 到 0.5862 和 0.0339-0.4994 之间。多态性信息含量在0.0333到0.3747之间。在这些 SNP 中，所有位点都被证实符合 Hardy-Weinberg 平衡（p  > 0.05）。本研究开发的新型SNPs将有助于太平洋蟾蜍的遗传保护和科学管理。