The present work compared two different routes in preparing oil palm trunk biomass (OPTB) hydrolysates for producing industrially-valuable succinic acid (SA). OPTB was initially pretreated with mild oxalic acid (1% w/v) under different reaction time to investigate its effect on the total sugar and inhibitor yields during pretreatment and subsequent enzymatic saccharification. Substantial solubilisation of hemicellulose (56–59 %) was achieved after pretreatment for 3−4 h. After enzymatic saccharification, high cellulose digestibility of 86–87 %, with 22.1–22.2 g sugar per 100 g of raw OPTB from washed pretreated solids (Route 1), and 71–74 %, with 43.2–44.9 g sugar per 100 g of raw OPTB from whole slurry (Route 2) saccharification, respectively was achieved. Fermentation of the hydrolysates derived from Route 2 by Actinobacillus succinogenes 130Z afforded a twofold increase in the SA production compared to that from Route 1. Mass balance calculations showed that 175 g of SA could be produced per kg of OPTB via Route 2 vs. 153 g via Route 1. The proposed approach in this study offers pioneering insights into the greatest improvement in SA yield achievable via efficient biomass utilisation.

目前的工作比较了制备油棕树干生物质 (OPTB) 水解物以生产具有工业价值的琥珀酸 (SA) 的两种不同途径。OPTB 最初用温和的草酸 (1% w/v) 在不同的反应时间进行预处理，以研究其在预处理和随后的酶糖化过程中对总糖和抑制剂产量的影响。预处理 3-4 小时后，半纤维素显着溶解（56-59%）。酶促糖化后，纤维素消化率为 86-87%，每 100 克来自洗涤过的预处理固体（路线 1）的原始 OPTB 含 22.1-22.2 克糖，71-74%，每 100 克含 43.2-44.9 克糖分别实现了来自全浆（路线 2）糖化的原始 OPTB。来自路线 2 的水解产物的发酵琥珀酸放线杆菌130Z，得到一个在SA生产增加两倍相比，从路线1质量平衡计算表明：175克SA的可每OPTB千克产生经由路线2与153克经由路线1.在此所提出的方法该研究为通过有效的生物质利用实现 SA 产量的最大提高提供了开创性的见解。