We describe a facile method to simultaneously measure the bending rigidity and capacitance of biomimetic lipid bilayers. Our approach utilizes the ellipsoidal deformation of quasi-spherical giant unilamellar vesicles induced by a uniform AC electric field. Vesicle shape depends on the electric field frequency and amplitude. Membrane bending rigidity can be obtained from the variation of the vesicle elongation on either field amplitude at fixed frequency or frequency at fixed field amplitude. Membrane capacitance is determined from the frequency at which the vesicle shape changes from prolate to oblate ellipsoid as the frequency is increased at a given field amplitude.

中文翻译：

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使用囊泡的电极形成仿生膜的机电表征

我们描述了一种同时测量仿生脂质双层的弯曲刚度和电容的简便方法。我们的方法利用了由均匀交流电场引起的准球形巨型单层囊泡的椭圆形变形。囊泡形状取决于电场频率和幅度。膜弯曲刚度可以从囊泡伸长率在固定频率下的场振幅或固定场振幅下的频率上的变化中获得。当频率在给定的场幅度下增加时，膜电容由囊泡形状从扁长形变为扁椭圆形的频率确定。