Accumulating evidence indicates that circular (circ)RNAs exhibit complex functions in diverse malignant tumors, including non‑small cell lung cancer (NSCLC). The role of the circRNA transcription adaptor 2A (circTADA2A) in NSCLC remains unclear. The expression, function and mechanism of circTADA2A in NSCLC development were investigated in the present study. The results revealed that circTADA2A was upregulated in NSCLC, and that knockdown of circTADA2A inhibited cell proliferation and migration in the NSCLC cell lines A549 and H1299. Functional assays demonstrated that circTADA2A promoted proliferation and migration via interacting with microRNA (miR)‑638. Bioinformatics and reverse transcription‑quantitative PCR assay confirmed that miR‑638 was expressed at low levels in NSCLC. In addition, it was found that miR‑638 served a tumor‑suppressive role and suppressed proliferation and migration via PCNA clamp associated factor (KIAA0101) inhibition in A549 and H1299 cells. Lastly, it was verified that circTADA2A promoted cell proliferation and migration, at least partially, via miR‑638/KIAA0101 signaling in A549 and H1299 cells. In summary, the present study showed that circTADA2A promoted NSCLC cell proliferation and migration via modulating miR‑638/KIAA0101 signaling.

中文翻译：

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环状 RNA TADA2A 通过调节 miR-638/KIAA0101 信号促进非小细胞肺癌的增殖和迁移。

越来越多的证据表明，环状（circ）RNA 在多种恶性肿瘤中表现出复杂的功能，包括非小细胞肺癌（NSCLC）。circRNA 转录接头 2A（circTADA2A）在 NSCLC 中的作用尚不清楚。本研究探讨了circTADA2A在NSCLC发展中的表达、功能和机制。结果显示 circTADA2A 在 NSCLC 中上调，并且 circTADA2A 的敲低抑制了 NSCLC 细胞系 A549 和 H1299 中的细胞增殖和迁移。功能分析表明，circTADA2A 通过与 microRNA (miR)-638 相互作用促进增殖和迁移。生物信息学和逆转录-定量 PCR 测定证实 miR-638 在 NSCLC 中低水平表达。此外，发现 miR-638 通过 A549 和 H1299 细胞中的 PCNA 钳夹相关因子 (KIAA0101) 抑制发挥肿瘤抑制作用并抑制增殖和迁移。最后，证实circTADA2A至少部分地通过A549和H1299细胞中的miR-638/KIAA0101信号传导促进细胞增殖和迁移。总之，本研究表明，circTADA2A 通过调节 miR-638/KIAA0101 信号传导促进 NSCLC 细胞增殖和迁移。