Pancreatic cancer (PC) is a lethal malignancy of the gastrointestinal tract. Previous studies have reported that microRNAs (miRNAs/miRs) are involved in the tumorigenesis of PC. Therefore, the present study aimed to determine the effects of miR‑7515 on PC cell proliferation, invasion and migration in vitro and in vivo, and investigate its underlying molecular mechanism using bioinformatics, double luciferase assay and western blotting. The results revealed that the expression levels of miR‑7515 were downregulated in PC, which predicted a poor clinical outcome. The overexpression of miR‑7515 significantly decreased the proliferation, invasive and migratory abilities of PC cells in vitro and in vivo, while the knockdown of miR‑7515 exerted the opposite effects. miR‑7515 was identified to directly bind to insulin‑like growth factor 1 (IGF‑1) and downregulate its expression, which subsequently downregulated the Ras/Raf/MEK/ERK signalling pathway. The overexpression of IGF‑1 reversed the inhibitory effects of miR‑7515 overexpression on PC cells. In conclusion, the findings of the present study indicated that miR‑7515 may act as a tumor suppressor in PC, as it repressed PC cell proliferation invasion and migration via downregulating the expression of IGF‑1 and the activity of the Ras/Raf/MEK/ERK signalling pathways.

中文翻译：

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miRNA-7515 通过下调 IGF-1 表达来抑制胰腺癌细胞增殖、迁移和侵袭。


胰腺癌（PC）是一种致命的胃肠道恶性肿瘤。以往的研究报道，microRNA（miRNA/miR）参与PC的肿瘤发生。因此，本研究旨在确定miR-7515对体外和体内PC细胞增殖、侵袭和迁移的影响，并利用生物信息学、双荧光素酶测定和蛋白质印迹研究其潜在分子机制。结果显示，PC 中 miR-7515 的表达水平下调，这预示着临床结果不佳。 miR-7515的过表达显着降低了PC细胞在体外和体内的增殖、侵袭和迁移能力，而miR-7515的敲低则产生相反的效果。 miR-7515 被鉴定为直接结合胰岛素样生长因子 1 (IGF-1) 并下调其表达，从而下调 Ras/Raf/MEK/ERK 信号通路。 IGF-1 的过表达逆转了 miR-7515 过表达对 PC 细胞的抑制作用。总之，本研究的结果表明 miR-7515 可能作为 PC 中的肿瘤抑制因子，因为它通过下调 IGF-1 的表达和 Ras/Raf/MEK 的活性来抑制 PC 细胞增殖、侵袭和迁移。 /ERK 信号通路。