To better explore the pathophysiology of FA and its therapy, we aimed to establish a simple and practicable FA model with Freund’s adjuvant and introduce an easy and reliable laboratory evaluation method for assessment of inflammation in intestinal segments at different anatomical locations. BALB/c mice were sensitized with ovalbumin combined with Freund’s adjuvant. Complete Freund’s adjuvant was chosen for the first sensitization and two weeks later incomplete Freund’s adjuvant was used for a second sensitization. Two weeks later, the sensitized mice were challenged with 50 mg ovalbumin every other day. After the 6 challenge, all mice were assessed for systemic anaphylaxis, and then sacrificed for sample collection. All sensitized mice showed anaphylactic symptoms and markedly increased levels of serum ovalbumin-specific IgE and IgG1. The activity of mast cell protease-1 (mMCPT-1) was significantly increased in the serum and interstitial fluid of the duodenum, jejunum, ileum, and colon. A successful FA model was established, of which inflammation occurred in the duodenum, jejunum, ileum, and colon. This model provides a reliable and simple tool for analysis of the mechanism of FA and methods of immunotherapy. Moreover, combined detection of ovalbumin-specific antibody and local mMCPT-1 levels could potentially be used as the major indicator for assessment of food allergy.

为了更好地探索 FA 的病理生理学及其治疗方法，我们旨在建立一个简单实用的弗氏佐剂 FA 模型，并引入一种简单可靠的实验室评估方法，用于评估不同解剖位置的肠段炎症。BALB/c小鼠用卵清蛋白联合弗氏佐剂致敏。第一次致敏选择完全弗氏佐剂，两周后使用不完全弗氏佐剂进行第二次致敏。两周后，每隔一天用 50 mg 卵清蛋白对致敏小鼠进行攻击。在第6次攻击后，评估所有小鼠的全身性过敏反应，然后处死以收集样品。所有致敏小鼠均表现出过敏症状，血清卵白蛋白特异性 IgE 和 IgG1 水平显着升高。十二指肠、空肠、回肠和结肠的血清和间质液中肥大细胞蛋白酶-1 (mMCPT-1) 的活性显着增加。建立成功的FA模型，炎症发生在十二指肠、空肠、回肠和结肠。该模型为分析 FA 的机制和免疫治疗方法提供了一种可靠且简单的工具。此外，卵清蛋白特异性抗体和局部 mMCPT-1 水平的联合检测可能被用作评估食物过敏的主要指标。该模型为分析 FA 的机制和免疫治疗方法提供了一种可靠且简单的工具。此外，卵清蛋白特异性抗体和局部 mMCPT-1 水平的联合检测可能被用作评估食物过敏的主要指标。该模型为分析 FA 的机制和免疫治疗方法提供了一种可靠且简单的工具。此外，卵清蛋白特异性抗体和局部 mMCPT-1 水平的联合检测可能被用作评估食物过敏的主要指标。