We studied the effect of technogenic radiation on the degree of promoter methylation in genes involved in apoptosis in blood lymphocytes of workers exposed to long-term γ-radiation during their professional activities. Blood samples for the analysis were obtained from 11 conventionally healthy men aged from 54 to 71 years (mean 66 years), workers of the Siberian Group of Chemical Enterprises working experience from 27 to 40 years (mean 30 years); the external exposure dose was 175.88 mSv (158.20-207.81 mSv). In all examined subjects, the degree of methylation of the promoters of apoptosis-related genes ranged from 0.22 to 50.00%. A correlation was found between the degree of methylation of BCLAF1 promoters (p=0.035) with the age of workers, BAX promoters (p=0.0289) with high content of aberrant cells, and APAF1 promoters (p=0.0152) with increased number of dicentric chromosomes. A relationship was found between the dose of external irradiation and the degree of methylation of gene promoters of BAD (p=0.0388), BID (р=0.0426), and HRK (р=0.0101) genes.

我们研究了技术辐射对在职业活动中长期暴露于γ射线的工人血液淋巴细胞凋亡相关基因启动子甲基化程度的影响。用于分析的血液样本来自 11 名年龄在 54 至 71 岁（平均 66 岁）、西伯利亚化学企业集团工作经验 27 至 40 年（平均 30 年）的常规健康男性；外照射剂量为175.88 mSv（158.20-207.81 mSv）。在所有检查对象中，凋亡相关基因启动子的甲基化程度在0.22%到50.00%之间。BCLAF1启动子的甲基化程度（p = 0.035）与工人年龄、BAX启动子（p = 0.0289）异常细胞含量高，APAF1启动子（p = 0.0152）双着丝粒染色体数量增加。外照射剂量与BAD（p =0.0388）、BID（р =0.0426）和HRK（р =0.0101）基因的基因启动子甲基化程度之间存在相关性。