Abstract

It was found that short exogenous peptides GlyGly and GlyAsp and the amino acid Gly can have a selective effect on the expression of genes for DNA-methyltransferases and SNF2 proteins from Nicotiana tabacum regenerants. It was noted that Gly and GlyGly decrease the expression of DNA methyltransferase genes (except for CMT3) and increase the expression of CMT3 by almost 30%. It was established that the presence of the acidic dipeptide GlyAsp in the nutrient medium increases the expression of genes not only of the CMT3 DNA methyltransferase but also of the supporting MET1 DNA methyltransferase as compared to the control variant. It was determined that all dipeptides and free glycine reduce (by two to four times) the expression of the DRM2 methyltransferase gene, which is responsible for DNA methylation de novo. It was shown that Gly, GlyGly, and GlyAsp modulate the expression of genes encoding the SNF2 family of proteins, decreasing the expression of almost all genes of the SNF2 family, only GlyGly increasing the expression of genes encoding an actin-dependent protein and CHR12. It was found that a decrease in the expression of the SNF2 genes leads to a decrease in the availability of free DNA for methylation, especially for de novo methylation.

中文翻译：

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Gly、GlyGly 和 GlyAsp 调节烟草烟草愈伤组织中 SNF2 家族基因和 DNA 甲基转移酶的表达

摘要

发现短外源肽 GlyGly 和 GlyAsp 以及氨基酸 Gly 可以选择性地影响来自烟草再生体的DNA 甲基转移酶和 SNF2 蛋白的基因表达。注意到 Gly 和 GlyGly 降低了 DNA 甲基转移酶基因（CMT3除外）的表达，并使 CMT3的表达增加了近 30%。已经确定，营养培养基中酸性二肽 GlyAsp 的存在增加了CMT3 DNA 甲基转移酶和支持性MET1基因的表达DNA甲基转移酶与对照变体相比。经测定，所有二肽和游离甘氨酸都降低（2 到 4 倍）DRM2甲基转移酶基因的表达，该基因负责 DNA从头甲基化。结果表明，Gly、GlyGly 和 GlyAsp 调节编码 SNF2 蛋白家族的基因的表达，降低了 SNF2 家族几乎所有基因的表达，只有 GlyGly 增加了编码肌动蛋白依赖性蛋白和 CHR12 的基因的表达。发现SNF2基因表达的降低导致甲基化的游离 DNA 可用性降低，尤其是从头甲基化。