The present experiment was conducted to breed a vulnerable cyprinid fish, Dawkinsia rohani (Rohan's barb), in captivity through hormonal inducement using one of the reproductive synthetic hormones WOVA-FH, since these fish did not breed spontaneously in captivity. The experiment was conducted in 500-L capacity fibre-reinforced plastic tanks with breeding hapa. The male (length 8.5 ± 1.5 cm and weight of 12 ± 2 g) and female (length 9.5 ± 2 cm and weight of 15 ± 1.5 g) brooders were injected intramuscularly with variable dose of WOVA-FH and released into the breeding hapa at 2:1 (male:female) ratio. Varied doses of WOVA-FH used for fish in the present study refer to 0.3 ml/kg (T1), 0.5 ml/kg (T2), 0.7 ml/kg (T3), 1.0 ml/kg (T4) and 0.0 ml/kg T0 (control). General courtship behaviour was observed in hormone-induced fish after 6–9 h after injection. Breeding was observed in all the treatment group fish except T0 (control groups). Success of spawning, fertilization and hatching rate varied depending on the dose of hormone. Significantly higher fecundity (1142.3 ± 40.20 eggs/female), fertilization rate (90.2 ± 0.67%) and hatching rate (88.92 ± 0.25%) were achieved in fish injected with 0.7 ml/kg for female and 0.3 ml/kg for male per kg body weight compared to other hormone treatment groups. Dosage levels influence the percentage of spawning, egg stripping, fertilization and hatching rate. The present study successfully standardized the dose for induced breeding of D. rohani. The standardized breeding technology will be helpful in breeding, conservation and propagation of the endemic indigenous ornamental fish, D. rohani.

中文翻译：

---

控制条件下成功繁殖地方性鲤科鱼类Dawkinsia rohani——初报

本实验旨在培育一种脆弱的鲤科鱼类Dawkinsia rohani（罗汉的倒钩），通过使用生殖合成激素 WOVA-FH 之一的激素诱导而被圈养，因为这些鱼在圈养中不会自发繁殖。实验是在 500 升容量的纤维增强塑料罐中进行的，带有繁殖箱。雄性（身长 8.5 ± 1.5 cm，体重 12 ± 2 g）和雌性（身长 9.5 ± 2 cm，体重 15 ± 1.5 g）育雏器被肌肉注射不同剂量的 WOVA-FH，并在2:1（男：女）比例。本研究中用于鱼的不同剂量的 WOVA-FH 是指 0.3 ml/kg (T1)、0.5 ml/kg (T2)、0.7 ml/kg (T3)、1.0 ml/kg (T4) 和 0.0 ml/kg kg T0（对照）。注射后 6-9 小时后，在激素诱导的鱼中观察到一般求爱行为。除T0（对照组）外，所有处理组鱼均观察到繁殖。产卵、受精和孵化率的成功取决于激素的剂量。注射 0.7 ml/kg 雌性鱼和 0.3 ml/kg 雄性鱼的鱼的繁殖力（1142.3 ± 40.20 个卵/雌性）、受精率（90.2 ± 0.67%）和孵化率（88.92 ± 0.25%）显着提高体重与其他激素治疗组相比。剂量水平影响产卵、卵剥离、受精和孵化率的百分比。本研究成功地标准化了诱导繁殖的剂量 与其他激素治疗组相比，男性每公斤体重 3 毫升/公斤。剂量水平影响产卵、卵剥离、受精和孵化率的百分比。本研究成功地标准化了诱导繁殖的剂量 与其他激素治疗组相比，男性每公斤体重 3 毫升/公斤。剂量水平影响产卵、卵剥离、受精和孵化率的百分比。本研究成功地标准化了诱导繁殖的剂量d。 鲁哈尼。标准化的养殖技术将有助于地方特有观赏鱼D 的选育、保藏和繁殖。 鲁哈尼。