Bacterial wilt (BW), caused by the soil-borne Ralstonia solanacearum, is a major disease in cultivated tomato (Solanum lycopersicum L.). Host resistance provides an environment-friendly and cost-effective strategy to control this disease. To investigate quantitative trait loci (QTL) for BW resistance, we conducted a genome-wide association study (GWAS) in a core collection of 191 tomato varieties. The 51 K Axiom® tomato array was used for genotyping and 38,541 confident SNPs were filtered for GWAS. We evaluated disease severity of the core collection in two independent seedling assays following inoculation with race 1 of R. solanacearum. A total of eight marker-trait associations (MTAs) for BW resistance was detected at P < 0.0005 using the compressed mixed linear model. Of these, the MTAs on chromosomes 4 and 12 were consistently found in both disease assays and their corresponding QTL (Bwr-4 and Bwr-12) explained 8.36–18.28% of total phenotypic variations. We also detected Bwr-6 on chromosome 6 at P < 0.0005 (the 1^st assay) and P < 0.01 (the 2^nd assay). Further analysis with 37 commercial F₁ cultivars demonstrated that the resulting SNP markers for these major QTL were effective in identifying resistant tomatoes. The cultivars with two of three major QTL showed higher levels of resistance relative to those with a single QTL. In addition, four MTAs on chromosomes 1 and 8–10 were found from only one of two assays, suggesting the presence of environment-specific QTL. These results facilitate genetic dissection of BW resistance and marker-assisted selection for developing elite cultivars in tomato breeding programs.

由土壤传播的青枯病菌引起的青枯病（BW）是栽培番茄（Solanum lycopersicum L.）的主要病害。宿主抗性为控制这种疾病提供了一种环境友好且具有成本效益的策略。为了研究 BW 抗性的数量性状基因座 (QTL)，我们在 191 个番茄品种的核心集合中进行了全基因组关联研究 (GWAS)。51 K Axiom® 番茄阵列用于基因分型，并过滤了 38,541 个可靠的 SNP 以进行 GWAS。我们在接种青枯菌的小种 1 后，在两个独立的幼苗试验中评估了核心收集物的疾病严重程度。在P检测到总共 8 个 BW 抗性的标记-性状关联 (MTA)< 0.0005 使用压缩混合线性模型。其中，染色体 4 和 12 上的 MTA 在两种疾病测定中都一致发现，它们相应的 QTL（Bwr-4和Bwr-12）解释了总表型变异的 8.36-18.28%。我们还在6号染色体上检测到Bwr-6，P < 0.0005（第^一次测定）和P < 0.01（^第二次测定）。进一步分析 37 架商用 F ₁栽培品种证明，这些主要 QTL 的 SNP 标记可有效识别抗性番茄。具有三个主要 QTL 中的两个的品种相对于具有单个 QTL 的品种表现出更高的抗性水平。此外，仅从两个测定中的一个中发现了染色体 1 和 8-10 上的四个 MTA，表明存在环境特异性 QTL。这些结果促进了 BW 抗性的遗传剖析和标记辅助选择，以在番茄育种计划中开发优良品种。