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Effects of Complex Electromagnetic Fields on Candida albicans Adhesion and Proliferation on Polyacrylic Resin
Applied Sciences ( IF 2.5 ) Pub Date : 2021-07-23 , DOI: 10.3390/app11156786
Morena Petrini , Silvia Di Lodovico , Giovanna Iezzi , Alessandro Cipollina , Adriano Piattelli , Luigina Cellini , Simonetta D’Ercole

(1) Background: The objectives of this study were to evaluate the effect of several sessions of the antibacterial protocol of complex electromagnetic fields (CMFs) on planktonic Candida albicans and fungal ability, after treatment with CMFs, to adhere and proliferate on acrylic resin materials. (2) Methods: Planktonic overnight cultures of Candida albicans were subjected to different entities of CMFs treatments. Four test groups were compared: “p1”: treated only with the first program of the antibacterial protocol; “p1–p5” subjected to the first five programs; “1 antibacterial” received one complete session of the protocol and “2 antibacterial” received two complete sessions. After the treatments, the number of colony forming units (CFUs) were recorded. Then, C. albicans broth cultures were cultivated on polyacrylic resin discs and evaluated for CFUs and subjected to scanning electron microscope (SEM) analysis. (3) Results: Microbiological analysis showed that CMFs promoted a significant reduction of C. albicans CFUs when the protocol “p1–p5” was applied. No statistically significant differences between test groups were observed if the time of exposure to CMFs was increased. SEM observations and CFUs showed that CMFs treatments have the ability to reduce C. albicans adherence and proliferation on discs. (4) Conclusions: The CMFs showed an antifungal effect as well as a decrease in C. albicans adhesion on polyacrylic resin.

中文翻译:

复合电磁场对白色念珠菌在聚丙烯树脂上的粘附和增殖的影响

(1) 背景:本研究的目的是评估复杂电磁场 (CMFs) 抗菌方案的几个阶段对浮游白色念珠菌和真菌能力的影响,在用 CMFs 处理后,在丙烯酸树脂材料上粘附和增殖. (2)方法:白色念珠菌浮游过夜培养物进行不同的CMFs处理。比较四个测试组:“p1”:仅用抗菌方案的第一个程序处理;“p1-p5”受前五个程序的影响;“1个抗菌”接受了一个完整的协议会话,“2个抗菌”接受了两个完整的会话。处理后,记录集落形成单位(CFU)的数量。然后,白色念珠菌肉汤培养物在聚丙烯酸树脂盘上培养并评估 CFU 并进行扫描电子显微镜 (SEM) 分析。(3) 结果:微生物学分析表明,当应用“p1-p5”方案时,CMF 促进了白色念珠菌CFU的显着减少。如果暴露于 CMF 的时间增加,则在测试组之间没有观察到统计学上的显着差异。SEM 观察和 CFU 表明 CMF 处理能够减少白色念珠菌在椎间盘上的粘附和增殖。(4) 结论:CMFs 显示出抗真菌作用以及减少白色念珠菌对聚丙烯酸树脂的粘附。
更新日期:2021-07-23
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