RNA-dependent RNA polymerase 1 delays the accumulation of viroids in infected plants,Molecular Plant Pathology

当前位置： X-MOL 学术 › Mol. Plant Pathol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

RNA-dependent RNA polymerase 1 delays the accumulation of viroids in infected plants
Molecular Plant Pathology ( IF 4.8 ) Pub Date : 2021-07-23 , DOI: 10.1111/mpp.13104
Shuai Li _{1,

2} , Zhixiang Zhang ₁ , Changyong Zhou ₂ , Shifang Li _{1,

3}

Affiliation

RNA-dependent RNA polymerase 1 (RDR1) is essential for plant antiviral defence, but its role in plant defence against viroid infection remains unknown. The present study aimed to identify the function and mechanism of RDR1 in plant resistance to viroid infection. Overexpression of Nicotiana tabacum RDR1 (NtRDR1) delayed the accumulation of potato spindle tuber viroid (PSTVd) genomic RNA and PSTVd-derived small RNA (sRNA) in Nicotiana benthamiana plants at the early invasion stage, but not in the late stage of infection. Conversely, virus-induced gene silencing of tomato RDR1 (SlRDR1a) increased the susceptibility to PSTVd infection (increased viroid accumulation). Salicylic acid (SA) pretreatment induced SlRDR1a expression and enhanced the defence against PSTVd infection in tomato plants. Our study demonstrated that RDR1 is involved in SA-mediated defence and restricts the early systemic invasion by PSTVd in plants. The decreased PSTVd accumulation in N. benthamiana was not caused by efficient accumulation of PSTVd sRNAs. These results deepen our understanding of the mechanism of RDR1 in plant defence responses to viroid attack.

中文翻译：

RNA依赖性RNA聚合酶1延迟受感染植物中类病毒的积累

RNA 依赖性 RNA 聚合酶 1 (RDR1) 对于植物抗病毒防御至关重要，但其在植物防御类病毒感染中的作用仍不清楚。本研究旨在明确RDR1在植物抵抗类病毒感染中的功能和机制。烟草RDR1 （ NtRDR1 ）的过度表达在入侵早期延迟了本塞姆氏烟草植物中马铃薯纺锤形块茎类病毒（PSTVd）基因组RNA和PSTVd衍生的小RNA（sRNA）的积累，但在感染后期却没有延迟。相反，病毒诱导的番茄RDR1 ( SlRDR1a ) 基因沉默增加了对 PSTVd 感染的易感性（增加了类病毒的积累）。水杨酸 (SA) 预处理诱导SlRDR1a表达并增强番茄植株对 PSTVd 感染的防御能力。我们的研究表明，RDR1 参与 SA 介导的防御并限制 PSTVd 在植物中的早期系统入侵。 N中 PSTVd 积累减少。 Benthamiana不是由 PSTVd sRNA 的有效积累引起的。这些结果加深了我们对 RDR1 在植物防御类病毒攻击中的机制的理解。

更新日期：2021-09-12

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11