Numerous serotypes which belong to the genus Enterovirus (EV) show variability in their virulence and clinical manifestations. They are also known to undergo changes caused by mutations and recombination during their circulation in the environment and the population. Various EV serotypes are prevalent in groundwater, wastewater and surface waters. Our previous studies showed that oral infection induces pancreatitis depending on specific conditions, such as gravidity, in an outbred murine model. Our aim in the present study was to further explore the pancreatic histopathology in an outbred mouse model following oral infection with clinical isolates from a patient who had aseptic meningitis and an isolate from a treated-sewage sample recovered from the residential area of the patient. The isolates were identified as coxsackievirus B4 (CVB4) in tissue culture. The CVB4 sewage-isolate induced pancreatitis after oral infection. In contrast, pancreatitis was absent following infection with the clinical isolates. Comparison of polyprotein sequences showed that the treated-sewage strains differed from the patient's isolates by 9 and 11 amino acids. We conclude that the isolates of clinical and environmental origin differed in their pathogenic properties and showed genetic variation.

中文翻译：

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柯萨奇病毒 B4 污水分离物诱导小鼠口腔感染后胰腺炎

属于肠道病毒属的多种血清型(EV) 显示其毒力和临床表现的变异性。众所周知，它们在环境和人群中的循环过程中会经历由突变和重组引起的变化。各种 EV 血清型普遍存在于地下水、废水和地表水中。我们之前的研究表明，在远交小鼠模型中，口腔感染会根据特定条件（例如妊娠）诱发胰腺炎。我们在本研究中的目的是进一步探索远交小鼠模型在口腔感染来自无菌性脑膜炎患者的临床分离株和从患者居住区回收的处理污水样本分离株后的胰腺组织病理学。这些分离株在组织培养中被鉴定为柯萨奇病毒 B4 (CVB4)。口腔感染后CVB4污水分离物诱发胰腺炎。相比之下，临床分离株感染后没有胰腺炎。多蛋白序列的比较表明，经过处理的污水菌株与患者的分离株有 9 和 11 个氨基酸的不同。我们得出结论，临床和环境来源的分离株的致病特性不同，并显示出遗传变异。