Lipids are highly dynamic molecules that, due to their hydrophobicity, are spatially confined to membrane environments. From these locations, certain privileged lipids serve as signaling molecules. For understanding the biological functions of subcellular pools of signaling lipids, induced proximity tools have been invaluable. These methods involve controlled heterodimerization, by either small-molecule or light triggers, of functional proteins. In the arena of lipid signaling, induced proximity tools can recruit lipid-metabolizing enzymes to manipulate lipid signaling and create artificial tethers between organelle membranes to control lipid trafficking pathways at membrane contact sites. Here, we review recent advances in methodology development and biological application of chemical-induced and light-induced proximity tools for manipulating lipid metabolism, trafficking, and signaling.

脂质是高度动态的分子，由于它们的疏水性，它们在空间上被限制在膜环境中。从这些位置，某些特权脂质充当信号分子。为了了解信号脂质亚细胞池的生物学功能，诱导接近工具非常宝贵。这些方法涉及通过小分子或光触发器对功能蛋白进行受控的异二聚化。在脂质信号传导领域，诱导接近工具可以招募脂质代谢酶来操纵脂质信号传导并在细胞器膜之间创建人工系链以控制膜接触位点的脂质运输途径。这里，