Mycorrhizal fungi are known to modulate the destructive effects of environmental stresses on plants. Arbuscular mycorrhizal fungi (AMF) symbiosis increases growth and stimulates the biosynthesis of bioactive compounds, thereby influencing polyphenolics content in medicinal plants under salt stress. The present study investigated the effects of AMF on growth traits, cell death, non-enzymatic antioxidants, and changes in the profile of phenolic compounds of Dracocephalum moldavica L. by HPLC analysis under different salinity concentrations. The experiment was performed as a factorial experiment based on a randomized complete block design including salinity treatment at four levels (0, 50, 100, and 150 mM NaCl) and mycorrhizal fungus treatment at three levels including non-inoculation and inoculated with Funneliformis mosseae and Claroideoglomus etunicatum with three replications. The experiment was performed in sterile controlled conditions in laboratory. The results showed that salinity reduced root colonization compared to the control conditions. Inoculation with AMF increased growth parameters and salinity tolerance of Deracocephalum Moldavica under all salinity levels. Through high-performance liquid chromatography, nine phenolic acids and flavonoids were analyzed in all treatments. Salinity and AMF inoculation altered the polyphenol profiles of Moldavian balm. F. mosseae inoculation increased most of the polyphenols at 50 and 150 mM NaCl, and C. etunicatum inoculation increased polyphenols at 100 mM NaCl more than F. mosseae. suggesting that F. mosseae and C. etunicatum mitigated the adverse effects of salinity, triggering non-enzymatic antioxidant activity by the accumulation of the flavonoid in roots and shoots and phenol in roots, enhancing growth parameters and reducing root cell death compared to non-mycorrhizal plants. As a positive point, salinity and mycorrhizal inoculation influenced the polyphenol content of D. moldavica and improved the production of phenolic compounds.

已知菌根真菌调节环境胁迫对植物的破坏性影响。丛枝菌根真菌 (AMF) 共生促进生长并刺激生物活性化合物的生物合成，从而影响盐胁迫下药用植物的多酚含量。本研究通过 HPLC 分析在不同盐度浓度下，研究了 AMF 对生长性状、细胞死亡、非酶抗氧化剂的影响以及Dracocephalum Moldavica L.酚类化合物谱的变化。该实验作为基于随机完整区组设计的析因实验进行，包括四个水平（0、50、100 和 150 mM NaCl）的盐度处理和三个水平的菌根真菌处理，包括未接种和接种Funneliformis mosseae和Claroideoglomus etunicatum具有三个重复。实验在实验室无菌控制条件下进行。结果表明，与对照条件相比，盐度降低了根系定植。在所有盐度水平下，接种 AMF 增加了摩尔达克氏菌的生长参数和盐度耐受性。通过高效液相色谱，在所有处理中分析了九种酚酸和黄酮类化合物。盐度和 AMF 接种改变了摩尔多瓦香脂的多酚谱。F. mosseae接种在 50 和 150 mM NaCl 时增加了大部分多酚，而C. etunicatum接种在 100 mM NaCl 时增加了多酚F.苔藓。表明F. mosseae和C. etunicatum减轻了盐度的不利影响，通过黄酮类化合物在根和芽中的积累以及根中的苯酚触发非酶抗氧化活性，与非菌根相比，提高了生长参数并减少了根细胞死亡植物。作为积极的一点，盐度和菌根接种影响了D. Moldavica的多酚含量并提高了酚类化合物的产量。