Abstract

The biochemical features that govern multi-functions of boiling soluble proteins are still not well documented. In this study, we have isolated and sequenced a water stress responsive cDNA encoding boiling soluble proteins designated as TaDreb, TaDHN and TaWCS20. (TaDREB: Triticum aestivum dehydration responsive element binding, TaDHN: Triticum aestivum dehydrin, and TaWCS120: Triticum aestivum cold acclimation protein WCS120) from seedlings exposed to water stress from wheat (PBW 175) cultivar tolerant to drought stress. We described in-silico analysis and molecular modelling of TaDreb, TaDHN and TaWCS20 through motif analysis, homology search, secondary structure prediction, active site prediction and 3D structure analysis. BLAST-N analysis revealed their resemblance to boiling soluble proteins encoding genes. The physio-chemical analysis revealed that TaDreb, TaDHN and TaWCS20 are low MW, hydrophilic, disordered and stable proteins. Multiple amino acid analysis revealed the presence of conserved domains responsive to water stress. 3D modelling analysis revealed the presence of structural rich motifs like beta hairpin, beta turns and bulges in a sequence dependent manner. Based upon these findings we suggest the possible mechanism that how these boiling soluble proteins preclude the unfavourable effect of drought stress.

中文翻译：

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来自小麦的水分胁迫响应性亲水性 TaDreb、TaDHN 和 TaWCS20 基因的 RT-PCR 扩增和计算机分析

摘要

控制煮沸可溶性蛋白质多功能的生化特征仍未得到充分记录。在这项研究中，我们已经分离并测序了一种对水分胁迫敏感的 cDNA，该 cDNA 编码被称为TaDreb、TaDHN和TaWCS20 的沸腾可溶性蛋白质。（TaDREB：普通小麦脱水响应元件结合，TaDHN：普通小麦脱水蛋白，和TaWCS120：普通小麦冷驯化蛋白 WCS120）来自暴露于耐干旱胁迫的小麦 (PBW 175) 栽培品种的水分胁迫的幼苗。我们描述了TaDreb的计算机分析和分子建模、TaDHN和TaWCS20通过基序分析、同源性搜索、二级结构预测、活性位点预测和 3D 结构分析。BLAST-N 分析揭示了它们与煮沸可溶性蛋白质编码基因的相似之处。理化分析表明，TaDreb、TaDHN和TaWCS20是低分子量、亲水性、无序和稳定的蛋白质。多氨基酸分析揭示了对水分胁迫有反应的保守结构域的存在。3D 建模分析揭示了结构丰富的图案的存在，如 β 发夹、β 转角和以序列相关的方式凸起。基于这些发现，我们提出了这些沸腾的可溶性蛋白质如何排除干旱胁迫的不利影响的可能机制。