The breakthrough in the field of nanotechnology has had a major influence on the field of biosensors. The integration of nanomaterials in the construction of biosensors could be related to analytical figures of merit such as lower detection limit, broad linear range, suitability for field research, and portability. In this line, nanomaterials have gained considerable attention in the fabrication of fluorescence-based biosensors. Significant research efforts have been made towards exploration and synthesis of various types of nanomaterials for subsequent use in the fluorescence-based biosensors. Nowadays, fluorescence biosensors have gained much attention among other types of biosensors due to high sensitivity, easy operation, multiplexed ability and non-destructive nature. The aim of this review is to identify key roles for nanomaterials in the design of fluorescence-based biosensors and to provide insight into the types and existence of nanomaterials. Nanomaterials may be used as a signal amplification probe, a fluorescence signal generator, and a fluorescence quencher. Furthermore, the utility of nano-fluorescence-based biosensors for the study of mycotoxins, antibiotic residues, and biomarkers has been demonstrated, as successful detection of these toxic molecules is critical.

纳米技术领域的突破对生物传感器领域产生了重大影响。纳米材料在生物传感器构建中的集成可能与分析品质因数有关，例如检测下限、线性范围宽、适用于现场研究和便携性。在这方面，纳米材料在基于荧光的生物传感器的制造中获得了相当大的关注。已经为探索和合成各种类型的纳米材料进行了大量的研究工作，以便随后在基于荧光的生物传感器中使用。目前，荧光生物传感器因其灵敏度高、操作简便、复用能力强、无损等优点而在其他类型的生物传感器中备受关注。本综述的目的是确定纳米材料在基于荧光的生物传感器设计中的关键作用，并深入了解纳米材料的类型和存在。纳米材料可用作信号放大探针、荧光信号发生器和荧光猝灭剂。此外，已经证明了基于纳米荧光的生物传感器在研究霉菌毒素、抗生素残留和生物标志物方面的效用，因为成功检测这些有毒分子至关重要。