The crop nitrogen and iron status play a key role for crop yield and quality in grapevines and other plant species. These elements are generally assessed by petiole tissue or destructive leaf analysis. Non-destructive estimation of nitrogen and iron sufficiency in grapevine leaves would contribute significantly to sensitive crop management. We therefore were recorded two different data against leaf nitrogen and iron content under the greenhouse for the ‘Sultana Seedless’ grape cultivar (values obtained from the SPAD-502 chlorophyll meter and Fe and N contents obtained from an atomic absorption spectrophotometer and micro-Kjeldahl method). Significant regression equations were determined between SPAD values and foliar Fe and N concentrations for Vitis vinifera L. cv. ‘Sultana Seedless’ grape cultivar during three consecutive years. The findings showed that there was a linear and positive correlation between total N and Fe content and SPAD-502 data. The intersection points varied between three years, but the slopes of the regression lines were similar in the results. At all sampling years the correlation of SPAD readings to N and Fe content were significant (r = 0.875* for 2012, r = 927* for 2013 and r = 0.925* for 2014 at Fe content and r = 0.909* for 2012, r = 0.865* for 2013, and r = 0.953* for 2014 at % N content, respectively). Therefore, it can be concluded that SPAD-502 could be used to estimate both iron and nitrogen content in grapevine leaves.

作物氮和铁的状态对葡萄藤和其他植物物种的作物产量和质量起着关键作用。这些元素通常通过叶柄组织或破坏性叶片分析进行评估。对葡萄叶片中氮和铁的充足性进行非破坏性评估将对敏感的作物管理做出重大贡献。因此，我们记录了温室下“Sultana Seedless”葡萄栽培品种的叶片氮和铁含量的两种不同数据（从 SPAD-502 叶绿素计获得的值以及从原​​子吸收分光光度计和微凯氏法获得的 Fe 和 N 含量）。确定了葡萄树的SPAD 值与叶面 Fe 和 N 浓度之间的显着回归方程 L.简历 连续三年获得“苏丹娜无核”葡萄品种。研究结果表明，总 N 和 Fe 含量与 SPAD-502 数据之间存在线性和正相关。三年之间的交点有所不同，但回归线的斜率在结果中是相似的。在所有采样年份，SPAD 读数与 N 和 Fe 含量的相关性均显着（ 2012 年为r = 0.875  *，2013 年为r = 927  *，2014 年为r = 0.925  *，2012 年为r = 0.909*，r  = 2013 年为 0.865*，r = 0.953*，分别为 2014 年的 % N 含量）。因此，可以得出结论，SPAD-502 可用于估计葡萄叶片中的铁和氮含量。