This study aimed to investigate the effects of Marigold flower powder (MFP) and marigold flower extract (MFEx) as feed additives on the performance, blood parameters, antioxidant capacity, immunological parameters, microbial content, digestive enzymes and digestibility in growing Japanese quail. A total number of 350 birds randomly distributed into seven groups, with five replicates of 10 birds each, control diet (control group), the second, third and fourth groups were fed on a basal diet within MFP 0.6%, 0.9% and 1.2% respectively. Fifth, sixth and seventh groups received the basal diet plus MFEx 150, 200 and 250 ppm respectively. Quails fed on a diet supplemented with MFEx 200 ppm had significantly higher lipase levels (p ≤ 0.001) compared to the control and MFP 0.6% without any significant difference with other experimental groups. Moreover, all digestibility coefficients (DC) of nutrients except for nitrogen-free extract were significantly (p ≤ 0.001) affected by all dietary treatments. Quails fed on the diet supplemented with MFEx 200 ppm significantly (p ≤ 0.001) presented the best body weight, body weight gain, feed conversion ratio and the lowest feed intake over all groups. Total lipid profile, kidney functions and liver functions were significantly affected by both MFP and MFEx diets. The group treated with MFEx 250 ppm had lowest E. coli and Salmonella population and the highest Lactobacilli population number. Antioxidant parameters and immune response (except for lymphocytes) were significantly affected (p ≤ 0.001) by different levels of MFP and MFEx. In conclusion, the addition of MFEx and MFP at 200 ppm followed by 250 ppm and 1.2% MFP, respectively, to the basal diet improved product productive performance, blood parameters, antioxidant capacity, immunological parameters, microbial content and digestibility in growing Japanese quail.

中文翻译：

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日粮添加金盏花粉和提取物（金盏花）对生长日本鹌鹑生产性能、营养消化率、血清生化、抗氧化参数和免疫反应的影响

本研究旨在研究万寿菊花粉（MFP）和万寿菊花提取物（MFEx）作为饲料添加剂对日本鹌鹑生长性能、血液参数、抗氧化能力、免疫学参数、微生物含量、消化酶和消化率的影响。总共350只鸡随机分为7组，每组10只鸡5个重复，对照日粮（对照组），第二、三、四组以MFP 0.6%、0.9%和1.2%以内的基础日粮饲喂分别。第五组、第六组和第七组分别接受基础饮食加 MFEx 150、200 和 250 ppm。喂食添加 MFEx 200 ppm 饮食的鹌鹑脂肪酶水平显着升高（p ≤ 0.001) 与对照组和 MFP 0.6% 相比，与其他实验组没有任何显着差异。此外，除无氮提取物外，所有营养物质的消化系数（DC）均 受到所有日粮处理的显着影响（p≤0.001）。饲喂添加 MFEx 200 ppm 日粮的鹌鹑显着 ( p  ≤ 0.001) 在所有组中表现出最佳的体重、体重增加、饲料转化率和最低的采食量。总血脂、肾功能和肝功能受到 MFP 和 MFEx 饮食的显着影响。用 MFEx 250 ppm 处理的组的E最低。 大肠杆菌和沙门氏菌种群和最高的乳酸杆菌人口数。不同水平的 MFP 和 MFEx显着影响抗氧化参数和免疫反应（淋巴细胞除外）（p  ≤ 0.001）。总之，在基础日粮中分别添加 200 ppm、250 ppm 和 1.2% MFP 的 MFEx 和 MFP，提高了生长日本鹌鹑的产品生产性能、血液参数、抗氧化能力、免疫学参数、微生物含量和消化率。