Enhancing the Stability of COVID-19 Serological Assay through Metal–Organic Framework Encapsulation,Advanced Healthcare Materials

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Enhancing the Stability of COVID-19 Serological Assay through Metal–Organic Framework Encapsulation
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2021-07-23 , DOI: 10.1002/adhm.202100410
Yixuan Wang ₁ , Zheyu Wang ₁ , Prashant Gupta ₁ , Jeremiah J Morrissey _{2,

3} , Rajesh R Naik ₄ , Srikanth Singamaneni _{1,

3}

Affiliation

Enzyme-linked immunosorbent assay is widely utilized in serologic assays, including COVID-19, for the detection and quantification of antibodies against SARS-CoV-2. However, due to the limited stability of the diagnostic reagents (e.g., antigens serving as biorecognition elements) and biospecimens, temperature-controlled storage and handling conditions are critical. This limitation among others makes biodiagnostics in resource-limited settings, where refrigeration and electricity are inaccessible or unreliable, particularly challenging. In this work, metal–organic framework encapsulation is demonstrated as a simple and effective method to preserve the conformational epitopes of antigens immobilized on microtiter plate under non-refrigerated storage conditions. It is demonstrated that in situ growth of zeolitic imidazolate framework-90 (ZIF-90) renders excellent stability to surface-bound SARS-CoV-2 antigens, thereby maintaining the assay performance under elevated temperature (40 °C) for up to 4 weeks. As a complementary method, the preservation of plasma samples from COVID-19 patients using ZIF-90 encapsulation is also demonstrated. The energy-efficient approach demonstrated here will not only alleviate the financial burden associated with cold-chain transportation, but also improve the disease surveillance in resource-limited settings with more reliable clinical data.

中文翻译：

通过金属有机框架封装增强 COVID-19 血清学检测的稳定性

酶联免疫吸附测定广泛应用于血清学测定，包括 COVID-19，用于检测和定量 SARS-CoV-2 抗体。然而，由于诊断试剂（例如，作为生物识别元件的抗原）和生物样本的稳定性有限，温控储存和处理条件至关重要。这种限制使得在资源有限的环境中进行生物诊断尤其具有挑战性，在这些环境中，制冷和电力无法获得或不可靠。在这项工作中，金属有机框架封装被证明是一种简单有效的方法，可以在非冷藏条件下保存固定在微量滴定板上的抗原的构象表位。结果表明，原位生长的沸石咪唑框架 90 (ZIF-90) 可为表面结合的 SARS-CoV-2 抗原提供出色的稳定性，从而在高温 (40 °C) 下保持检测性能长达 4 周。作为一种补充方法，还证明了使用 ZIF-90 封装来保存 COVID-19 患者的血浆样本。这里展示的节能方法不仅可以减轻与冷链运输相关的经济负担，而且可以通过更可靠的临床数据改善资源有限环境中的疾病监测。

更新日期：2021-09-22

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