Shrimp allergy is a growing problem among the European population. TSLP, IL-25 and IL-33 are involved in the pathophysiology of allergic diseases, including asthma and atopic dermatitis, as they activate the Th2-dependent immune response. Thirty-seven patients (18 male and 19 female) with a positive history of symptoms associated with shrimp consumption were selected. All patients had blood samples taken to assess the concentration of allergen-specific IgE (sIgE) to house dust mites (HDM) and shrimp (Singleplex, quantitative method with cut off value > 0,35 kAU/L) as well as the level of allergen components using the ImmunoCap ISAC method (Microarray test, semi-quantitative with cut off value > 0,3 ISU-E). The concentrations of TSLP, IL-25 and IL-33 in the patients’ blood serum was assessed using the ELISA method (Cusabio). Twenty patients with negative allergy history of allergic disease tests were included in the control group. Among the 37 shrimp-allergic patients, ImmunoCap ISAC was identified the presence of sIgE to the available shrimp allergen components in only 14 cases (37.8%). TSLP and IL25 levels were significantly higher in the study group. No statistically significant correlation was found between the concentration of analyzed alarmins and the concentration of sIgE level to shrimp or HDM between the study and control groups. No statistically significant correlation was found between poly-sensitization occurring in patients and levels of TSLP, IL-25 and IL-33 . In shrimp-allergic patients, the concentrations of TSLP and IL-25 were significantly higher than in the control group (1.33 vs. 0.49 and 157 vs. 39.36, respectively). There was no correlation between the concentrations of TSLP, IL-25 and IL-33 and the concentration of sIgE in the patients or the number of allergen components that the patients were sensitized to. Trial registration: Bioethics Committee 147/2015, 11.03.2015.

中文翻译：

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对虾过敏患者的 TSLP、IL 25 和 IL 33 评估

虾过敏是欧洲人口中日益严重的问题。TSLP、IL-25 和 IL-33 参与过敏性疾病的病理生理学，包括哮喘和特应性皮炎，因为它们激活 Th2 依赖性免疫反应。选择了 37 名患者（18 名男性和 19 名女性）具有与虾消费相关的阳性症状史。所有患者均采集血液样本以评估对屋尘螨 (HDM) 和虾的过敏原特异性 IgE (sIgE) 浓度（单重，截止值 > 0.35 kAU/L 的定量方法）以及使用 ImmunoCap ISAC 方法（微阵列测试，半定量，截止值 > 0,3 ISU-E）检测过敏原成分。使用ELISA方法（Cusabio）评估患者血清中TSLP、IL-25和IL-33的浓度。20名过敏性疾病试验阴性过敏史的患者被纳入对照组。在 37 名虾过敏患者中，ImmunoCap ISAC 仅在 14 例 (37.8%) 中发现对可用虾过敏原成分的 sIgE 存在。研究组的 TSLP 和 IL25 水平显着更高。在研究组和对照组之间，在分析的警报蛋白浓度与对虾或 HDM 的 sIgE 水平浓度之间没有发现统计学上显着的相关性。在患者中发生的多敏化与 TSLP、IL-25 和 IL-33 水平之间没有发现统计学上显着的相关性。在虾过敏患者中，TSLP 和 IL-25 的浓度显着高于对照组（分别为 1.33 对 0.49 和 157 对 39.36）。TSLP、IL-25和IL-33的浓度与患者体内sIgE的浓度或患者致敏的过敏原成分的数量之间没有相关性。试验注册：生物伦理委员会 147/2015, 11.03.2015。