Equiseti herba has been traditionally indicated in bacterial diseases of the efferent urinary tract or bad healing wounds in many regions worldwide. Most of the plant material used for medical purposes comes from collections of wild growing plants. The European Pharmacopoeia requires that Equiseti herba should consist of a minimum of 95% Equisetum arvense and a maximum of 5% foreign ingredients. This includes Equisetum palustre, which is known for its potentially toxic alkaloid palustrine. However, both Equisetum species are quite common, look morphologically very similar, and share similar habitats, hence, are therefore often confused. Recently, several structurally related Equisetum alkaloids have been identified in E. palustre but not in E. arvense. We have established a hydrophilic interaction liquid chromatography HPLC-ESI-MS/MS method for the detection of these E. palustre-specific Equisetum alkaloids in order to quantify the contamination of Equiseti herba (E. arvense) by E. palustre plant material. In a second, independent approach, the results of the HPLC-MS/MS analysis were confirmed by scanning electron microscopy, looking for the species-specific characteristics of the stoma apparatus of E. palustre. Thirty-four Equiseti herba products obtained from different pharmacies, drug stores, supermarkets, and web stores were analyzed. The majority of the products (26 out of 34) were Equisetum alkaloid positive, with contents ranging from 0.29 – 21.7 mg of Equisetum alkaloids/kg (d. w.). In addition, the transfer of Equisetum alkaloids into tea infusions was investigated, demonstrating a 42 to 60% transfer rate for cold and hot water extraction of Equisetum alkaloid-contaminated Equiseti herba, respectively.

中文翻译：

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使用 HPLC-MS/MS 基于生物标志物测定木贼草污染

Equiseti Herba 传统上被用于治疗传出尿道的细菌性疾病或世界许多地区愈合不良的伤口。大多数用于医疗目的的植物材料来自野生植物的集合。欧洲药典要求木贼草应由最少 95% 的木贼和最多 5% 的外国成分组成。这包括 Equisetum palustre，它以其潜在有毒的生物碱沼泽而闻名。然而，这两种木贼属很常见，在形态上看起来非常相似，并且共享相似的栖息地，因此经常被混淆。最近，已在 E. palustre 但未在 E. arvense 中鉴定出几种结构相关的木贼生物碱。我们已经建立了一种亲水相互作用液相色谱 HPLC-ESI-MS/MS 方法来检测这些 E. palustre 特异性木贼生物碱，以量化 E. palustre 植物材料对木贼草 (E. arvense) 的污染。在第二种独立的方法中，HPLC-MS/MS 分析的结果通过扫描电子显微镜得到证实，寻找 E. palustre 造口装置的物种特异性特征。分析了从不同药店、药店、超市和网上商店获得的 34 种木犀草产品。大部分产品（34 种产品中的 26 种）为木贼生物碱阳性，含量为 0.29 – 21.7 毫克木贼生物碱/公斤（d. w.）。此外，研究了木贼属生物碱向茶浸液中的转移，