Flueggea suffruticosa is a traditional Chinese medicine that has been commonly used for the treatment of inflammatory ailments, including rheumatism and lumbago. Suffrutines A and suffrutines B are a pair of novel E,E and Z,E isomeric indolizidine alkaloids isolated from the roots of F. suffruticosa. However, their anti-inflammatory activity has not been reported thus far. The aim of this study was to investigate the inhibitory effect of inflammatory mediators and possible mechanisms of suffrutines A and B in lipopolysaccharide-induced RAW264.7 cells. Results showed that suffrutines A and B could remarkably inhibit the production of nitric oxide, prostaglandin E2, interleukin-6, inducible nitric oxide synthase, and cyclooxygenase-2 in lipopolysaccharide-induced RAW264.7 cells. Further evaluation demonstrated that compared with suffrutines A, suffrutines B could more significantly inhibit the phosphorylation of IKKα/β, the degradation of IκBα, and the nuclear translocation of the p65 and p52 subunits in the canonical and non-canonical nuclear factor-κB pathways. Therefore, suffrutines B exhibited more potent inhibitory activity on inflammatory mediators than suffrutines A.

中文翻译：

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Suffrutines A 和 B 通过抑制 NF-κB 信号通路抑制 LPS 诱导的 RAW264.7 细胞中炎症介质的表达

Flueggea suffruticosa 是一种传统中药，常用于治疗炎症性疾病，包括风湿病和腰痛。Suffrutines A 和 suffrutines B 是从 F. suffruticosa 的根中分离出来的一对新型 E,E 和 Z,E 异构吲哚里西啶生物碱。然而，迄今为止尚未报道它们的抗炎活性。本研究的目的是探讨炎症介质的抑制作用以及糖苷脂A和B在脂多糖诱导的RAW264.7细胞中的可能机制。结果表明，suffrutines A和B能显着抑制脂多糖诱导的RAW264.7细胞中一氧化氮、前列腺素E2、白细胞介素6、诱导型一氧化氮合酶和环氧合酶2的产生。进一步的评估表明，与舒夫丁 A 相比，suffrutines B 可以更显着地抑制经典和非经典核因子-κB 通路中 IKKα/β 的磷酸化、IκBα 的降解以及 p65 和 p52 亚基的核转位。因此，suffrutines B 对炎症介质的抑制活性比 suffrutines A 更强。