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Characterization of a novel botoulivirus isolated from the phytopathogenic fungus Sclerotinia sclerotiorum
Archives of Virology ( IF 2.5 ) Pub Date : 2021-07-22 , DOI: 10.1007/s00705-021-05168-x
Fan Mu 1 , Jichun Jia 1 , Yunxiang Xue 1 , Daohong Jiang 1 , Yanping Fu 1 , Jiasen Cheng 1 , Yang Lin 1 , Jiatao Xie 1
Affiliation  

Sclerotinia sclerotiorum ourmiavirus 17 (SsOV17) was isolated from the hypovirulent strain GF3 of Sclerotinia sclerotiorum. The genome of SsOV17 is 2,802 nt in length and contains a single long open reading frame (ORF) flanked by a short structured 5ʹ-untranslated region (5ʹ-UTR) (28 nt) and a long 3ʹ-UTR (788 nt), respectively. The ORF encodes a protein with 663 amino acids and a predicted molecular mass of 75.0 kDa. A BLASTp search indicated that the protein encoded by SsOV17 is closely related to the putative RNA-dependent RNA polymerase (RdRp) of Sclerotinia sclerotiorum ourmiavirus 13 (71% identity). A multiple sequence alignment indicated that eight conserved amino acid motifs were present in the RdRp conserved region of SsOV17. Phylogenetic analysis demonstrated that SsOV17 clustered with members of the genus Botoulivirus.



中文翻译:

从植物病原真菌核盘菌中分离的新型肉毒杆菌病毒的表征

菌核病菌ourmiavirus 17 (SsOV17) 分离自核盘菌的低毒株GF3. SsOV17 的基因组长度为 2,802 nt,包含一个长开放阅读框 (ORF),其两侧分别是一个短结构的 5ʹ-非翻译区 (5ʹ-UTR) (28 nt) 和一个长 3ʹ-UTR (788 nt)。 . ORF 编码具有 663 个氨基酸和预测分子量为 75.0 kDa 的蛋白质。BLASTp 搜索表明,由 SsOV17 编码的蛋白质与核盘菌核盘菌病毒 13 的假定 RNA 依赖性 RNA 聚合酶 (RdRp) 密切相关(71% 同一性)。多序列比对表明 SsOV17 的 RdRp 保守区域中存在八个保守的氨基酸基序。系统发育分析表明,SsOV17 与肉毒杆菌属的成员聚集在一起。

更新日期:2021-09-07
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