A novel virus with a quadruple genome of negative-sense, single-stranded RNA was identified by high-throughput sequencing (HTS) in a grass sample from Saxony-Anhalt, Germany, and tentatively called Festuca stripe-associated virus (FSaV). The genome of FSaV consists of four segments and a total of 16,535 nucleotides (nt) which encode seven open reading frames (ORF). FSaV shares highest nt identity (between 72.84% to 80.74%) to Iranian wheat stripe virus (IWSV) and rice hoja blanca virus (RHBV). Additionally, pairwise comparisons between the amino acid sequences of the ORFs on the genome of FSaV and the corresponding ones on the genomes of the members of the Tenuvirus genus showed that FSaV shared 83.17% and 90.85% (amino acid) aa identity to IWSV. Moreover, the non-coding intergenic regions (ncIR) shared only between 49.5% to 60.87% nt identity to the corresponding regions on the IWSV genome. Based on the ICTV species demarcation, the results suggest that FSaV may represent a new species of the genus Tenuivirus. Plastid sequence analysis of the HTS data showed that the original host is a member of the genus Festuca most likely the species Festuca pratensis.

通过高通量测序 (HTS) 在来自德国萨克森-安哈尔特的草样本中鉴定出一种具有四重负义单链 RNA 基因组的新型病毒，暂称为羊茅条纹相关病毒 (FSaV)。FSaV 的基因组由四个片段和总共 16,535 个核苷酸 (nt) 组成，它们编码七个开放阅读框 (ORF)。FSaV 与伊朗小麦条纹病毒 (IWSV) 和水稻霍哈布兰卡病毒 (RHBV) 具有最高的 nt 同一性（在 72.84% 至 80.74% 之间）。此外，FSaV基因组上ORF的氨基酸序列与Tenuvirus成员基因组上的相应氨基酸序列之间的成对比较属显示 FSaV 与 IWSV 共享 83.17% 和 90.85%（氨基酸）的 aa 同一性。此外，非编码基因间区域（ncIR）与 IWSV 基因组上相应区域的 nt 同一性仅在 49.5% 至 60.87% 之间共享。基于 ICTV 种属划分，结果表明 FSaV 可能代表了细纹病毒属的一个新种。HTS数据的质体序列分析表明，原始宿主是羊茅属的成员，最有可能是羊茅属的物种。