ABSTRACT

The purpose of this study was to investigate and compare the antioxidant and cytotoxic activities of Sarcosphaera crassa and Tricholoma terreum extracts. Phenolic compounds analysis of both mushroom extracts has been carried out by HPLC. Antioxidant activities of the extracts were tested by using in vitro assays. The cytotoxicity of extracts on HT-29 cell line were determined by the WST assay. The total phenolic and total flavonoid contents of T. terreum extracts were higher than those S. crassa. Also, we detected that T. terreum extracts had higher total antioxidant activity and scavenging ability on DPPH radicals than S. crassa extracts. However, the T. terreum methanol extracts showed 27% cytotoxic effect in HT-29 cell line whereas the ethanolic extracts of S. crassa showed cytotoxicity 45%. These results indicate that both mushroom extracts have considerable cytotoxic and antioxidant properties and may be utilized as a promising source of therapeutics.

摘要

本研究的目的是研究和比较粗糙肉球菌和土口蘑提取物的抗氧化和细胞毒活性。两种蘑菇提取物的酚类化合物分析均通过 HPLC 进行。通过使用体外测定法测试提取物的抗氧化活性。通过 WST 测定法测定提取物对 HT-29 细胞系的细胞毒性。T. terreum提取物的总酚和总黄酮含量高于S. crassa。此外，我们检测到T. terreum提取物比S. crassa提取物具有更高的总抗氧化活性和对 DPPH 自由基的清除能力。但是，那T. terreum甲醇提取物在 HT-29 细胞系中显示出 27% 的细胞毒性作用，而粗糙链霉菌的乙醇提取物显示出 45% 的细胞毒性作用。这些结果表明，两种蘑菇提取物都具有相当大的细胞毒性和抗氧化特性，可用作有前景的治疗来源。