Faster, better, and cheaper: harnessing microfluidics and mass spectrometry for biotechnology,RSC Chemical Biology

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Faster, better, and cheaper: harnessing microfluidics and mass spectrometry for biotechnology
RSC Chemical Biology ( IF 4.2 ) Pub Date : 2021-07-20 , DOI: 10.1039/d1cb00112d
Noel S Ha _{1,

2} , Markus de Raad ₃ , La Zhen Han _{3,

4} , Amber Golini _{3,

4} , Christopher J Petzold _{1,

2} , Trent R Northen _{1,

2,

3,

4}

Affiliation

High-throughput screening technologies are widely used for elucidating biological activities. These typically require trade-offs in assay specificity and sensitivity to achieve higher throughput. Microfluidic approaches enable rapid manipulation of small volumes and have found a wide range of applications in biotechnology providing improved control of reaction conditions, faster assays, and reduced reagent consumption. The integration of mass spectrometry with microfluidics has the potential to create high-throughput, sensitivity, and specificity assays. This review introduces the widely-used mass spectrometry ionization techniques that have been successfully integrated with microfluidics approaches such as continuous-flow system, microchip electrophoresis, droplet microfluidics, digital microfluidics, centrifugal microfluidics, and paper microfluidics. In addition, we discuss recent applications of microfluidics integrated with mass spectrometry in single-cell analysis, compound screening, and the study of microorganisms. Lastly, we provide future outlooks towards online coupling, improving the sensitivity and integration of multi-omics into a single platform.

中文翻译：

更快、更好、更便宜：利用微流体和质谱进行生物技术

高通量筛选技术广泛用于阐明生物活性。这些通常需要权衡测定特异性和灵敏度以实现更高的通量。微流控方法能够快速操作小体积，并在生物技术中得到了广泛的应用，可以改善对反应条件的控制，加快测定速度并减少试剂消耗。质谱与微流体的集成有可能创建高通量、灵敏度和特异性检测。本文介绍了广泛使用的质谱电离技术，这些技术已成功地与微流控方法集成，例如连续流系统、微芯片电泳、液滴微流控、数字微流控、离心微流控和纸微流控。此外，我们还讨论了微流控与质谱相结合在单细胞分析、化合物筛选和微生物研究中的最新应用。最后，我们对在线耦合、提高多组学敏感性以及将多组学集成到单一平台中的未来前景进行了展望。

更新日期：2021-07-22

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