The data with regards to the regional variants of distinct HPV types is of great value. Accordance with this, this study aimed to investigate the sequence variations of E6 gene and long control region of HPV 39 among normal, premalignant and malignant cervical samples in order to characterize the frequent HPV 39 variants circulating in Tehran, Iran. In total, 70 cervical samples (45 normal, 16 premalignant, and 9 malignant samples) infected with HPV 39 were analyzed by nested-PCR and sequencing. Our results revealed that all samples belonged to A lineage. Almost all sequences (98.6%) were classified in A1 sublineage and only one sample (1.4%) was A2 sub lineage. Our findings showed that lineages A, sublineage A1, is dominant in Tehran, Iran. However, the small sample size was the most important limitations of this study. Further studies with larger sample size from different geographical regions of Iran are necessary to estimate the pathogenicity risk of HPV 39 variants in this population.

中文翻译：

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伊朗妇女宫颈样本中 39 型人乳头瘤病毒的谱系分析

关于不同 HPV 类型的区域变异的数据非常有价值。据此，本研究旨在调查正常、癌前和恶性宫颈样本中 E6 基因和 HPV 39 长控制区的序列变异，以表征伊朗德黑兰流行的常见 HPV 39 变体。总共有 70 个宫颈样本（45 个正常样本、16 个癌前样本和 9 个恶性样本）感染了 HPV 39，通过巢式 PCR 和测序进行了分析。我们的结果表明，所有样本都属于 A 谱系。几乎所有序列 (98.6%) 都归类为 A1 亚谱系，只有一个样本 (1.4%) 是 A2 亚谱系。我们的研究结果表明，谱系 A，亚谱系 A1，在伊朗德黑兰占主导地位。然而，小样本量是本研究最重要的局限性。