Affinity monolith chromatography (AMC) is a liquid chromatographic technique that utilizes a monolithic support with a biological ligand or related binding agent to isolate, enrich, or detect a target analyte in a complex matrix. The target-specific interaction exhibited by the binding agents makes AMC attractive for the separation or detection of a wide range of compounds. This article will review the basic principles of AMC and recent developments in this field. The supports used in AMC will be discussed, including organic, inorganic, hybrid, carbohydrate, and cryogel monoliths. Schemes for attaching binding agents to these monoliths will be examined as well, such as covalent immobilization, biospecific adsorption, entrapment, molecular imprinting, and coordination methods. An overview will then be given of binding agents that have recently been used in AMC, along with their applications. These applications will include bioaffinity chromatography, immunoaffinity chromatography, immobilized metal-ion affinity chromatography, and dye-ligand or biomimetic affinity chromatography. The use of AMC in chiral separations and biointeraction studies will also be discussed.

中文翻译：

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亲和整体层析：一般原理和最新发展回顾


亲和整体柱色谱 (AMC) 是一种液相色谱技术，利用带有生物配体或相关结合剂的整体支撑物来分离、富集或检测复杂基质中的目标分析物。结合剂所表现出的靶标特异性相互作用使得 AMC 对于分离或检测多种化合物具有吸引力。本文将回顾AMC的基本原理以及该领域的最新进展。将讨论 AMC 中使用的载体，包括有机、无机、混合、碳水化合物和冷冻凝胶整体。还将研究将结合剂附着到这些整体上的方案，例如共价固定、生物特异性吸附、包埋、分子印迹和配位方法。然后将概述最近在 AMC 中使用的结合剂及其应用。这些应用将包括生物亲和层析、免疫亲和层析、固定金属离子亲和层析以及染料配体或仿生亲和层析。还将讨论 AMC 在手性分离和生物相互作用研究中的应用。