Abstract

“Pitanga” leaf hydroethanolic extract (PLHE) presents high antioxidant and antimicrobial activities. The encapsulation of this extract in W/O/W double emulsions could protect its bioactive properties. The objective of this research was to encapsulate PLHE in the inner W phase of a double emulsion and analyze the emulsifiers concentrations and phase ratios effects on its droplet size and the physical stability. Moreover, the double emulsion rheological behavior, antioxidant and antimicrobial activities in their more stable formulation was investigated. For comparison purpose, antioxidant and antimicrobial activities of the PLHE was also determined. Overall, the droplet size and stability were affected by the concentration of emulsifiers and phase ratios. The double emulsion presented heterogeneous droplet size, good physical stability and non-Newtonian behavior. PLHE presented high antioxidant and antimicrobial activities, which was maintained after processing to encapsulation into double emulsion. In conclusion, it was possible to produce active and stable double emulsion loaded with PLHE.

摘要

“Pitanga”叶水乙醇提取物 (PLHE) 具有很高的抗氧化和抗菌活性。将该提取物包封在 W/O/W 双乳液中可以保护其生物活性。本研究的目的是将PLHE封装在双乳液的内W相中，并分析乳化剂浓度和相比对其液滴尺寸和物理稳定性的影响。此外，还研究了其更稳定的配方中的双乳液流变行为、抗氧化和抗菌活性。为进行比较，还测定了 PLHE 的抗氧化和抗菌活性。总体而言，液滴大小和稳定性受乳化剂浓度和相比的影响。双乳液呈现不均匀的液滴大小，良好的物理稳定性和非牛顿行为。PLHE表现出很高的抗氧化和抗菌活性，在加工成双乳液后仍能保持这种活性。综上所述，可以生产出活性稳定的载有PLHE的双乳液。