Abstract

Tea tree oil (TTO) is a broad-spectrum natural antimicrobial agent. Its application is limited due to its volatile property. To reduce its volatility and improve its sustained-release performance, a synergistic sustained-release system was prepared. Montmorillonite (Mt) was ion-exchanged with sodium ions (Na⁺) and zinc ions (Zn²⁺), then organically modified with cetyl trimethyl ammonium bromide (CTAB) and chitosan (CS) to improve its sustained release performance. The results indicated that Mt was exfoliated, but still maintained its ordered layer structure. The surface of CS-Zn-OMt was rough, because some pores and surfaces were filled. The addition of Zn²⁺ increased the loading of CS (37.8 − 40.8%) and induced more uniform distribution of CS on Mt. Also, CS-Zn-OMt achieved a high loading capacity for TTO and the loading value was 222.0 mg*g⁻¹. Furthermore, the release studies showed that CS-Zn-OMt exhibited sustained-release behavior due to the release from the outer layer of CS and the Mt channel simultaneously. The results of antibacterial test showed that the addition of CS inhibited the drift of Mt and showed good antibacterial performance because of strong effective drug-carrier interaction. The developed system can be potentially applied for the delivery of volatile essential oils for antibacterial infection.

摘要

茶树油 (TTO) 是一种广谱天然抗菌剂。由于其挥发性，其应用受到限制。为降低其挥发性，提高其缓释性能，制备了协同缓释体系。^{蒙脱石 (Mt) 与钠离子 (Na +} ) 和锌离子 (Zn ²⁺ )进行离子交换，然后用十六烷基三甲基溴化铵 (CTAB) 和壳聚糖 (CS) 进行有机改性，以提高其缓释性能。结果表明，山被剥落，但仍保持其有序层结构。CS-Zn-OMt 的表面很粗糙，因为一些孔隙和表面被填充了。^{Zn 2+}的加入增加了 CS 的负载量 (37.8 − 40.8%) 并诱导 CS 在 Mt 上的分布更均匀。此外，CS-Zn-OMt 实现了 TTO 的高负载能力，负载值为 222.0 mg*g ^-1。此外，释放研究表明，由于同时从 CS 外层和 Mt 通道释放，CS-Zn-OMt 表现出缓释行为。抗菌试验结果表明，CS的加入抑制了Mt的漂移，由于有效的药物-载体相互作用强，表现出良好的抗菌性能。所开发的系统可潜在地用于输送挥发性精油以抗菌感染。