To clarify the histopathological characteristics of Camellia oleifera that were infected by Colletotrichum fructicola, this study utilized frozen tissue sections, whole-stain clearing and transmission electron microscopy to observe the time of transformation from biotrophic to necrotrophic when Co. fructicola infects leaves, as well as the colonization and expansion of the pathogenic fungus at different stages on the leaves. The Co. fructicola conidia produced germ tubes 2 h post-inoculation, and appressoria began to form 4 h after inoculation. There were two ways that pathogens can use to invade the leaves of Ca. oleifera. One was to directly penetrate the host cuticle, while the other involved invasion from the stomata of the host using germ tubes or hyphae. A few infection vesicles could then be observed in the leaf tissue, and the infection vesicles produce thicker septal primary hyphae after 24 h of infection. When the infection reached the 40 h point, the fungus started to produce secondary hyphae with smaller diameter than that of the primary hyphae and gradually expanded into the adjacent cells. Transmission electron microscopy revealed that when the secondary hyphae entered the mesophyll cells, the contents in the cells were digested and a cavity gradually formed, indicating that the cells had been killed. Thus, the appearance of the secondary hyphae marked the point at which Co. fructicola entered into a destructive necrotrophic stage.

为了澄清的组织病理学特征油茶是由感染炭疽病腐病菌，本研究中使用冷冻组织切片，全染色清算和透射电镜从活体营养的观察转型的时候死体的时候有限公司腐病菌感染树叶，以及病原真菌在不同阶段在叶片上的定植和扩张。的有限腐病菌的分生孢子产生芽管2小时接种后，和附着胞开始形成接种后4小时。病原体可以通过两种方式入侵Ca的叶子。油茶. 一种是直接穿透宿主角质层，而另一种涉及使用胚管或菌丝从宿主的气孔侵入。然后在叶组织中可以观察到一些感染小泡，感染小泡在感染 24 小时后产生更厚的间隔初级菌丝。当感染达到 40 h 时，真菌开始产生直径小于初级菌丝的次级菌丝，并逐渐扩展到相邻的细胞中。透射电镜显示，当次生菌丝进入叶肉细胞时，细胞内的内容物被消化，逐渐形成空腔，表明细胞已被杀死。因此，次生菌丝的出现标志着Co. fructicola 进入破坏性坏死阶段。