Photo-chemotherapy (PCT) reveals great potential in hepatocellular carcinoma (HCC) treatment, therefore the construct of smart PCT nano-agents with high photothermal conversion efficiency and accurate drug delivery is of great significant. Herein, a novel hybrid nanomaterial MGO-TCA-FA has been designed and constructed by grafting the triformyl cholic acid (TCA) and folic acid (FA) on the surface of Fe₃O₄ modified graphene oxide (MGO). The doxorubicin hydrochloride (DOX) as a model drug could be effectively loaded on the MGO-TCA-FA via hydrogen bonding and π-π stacking (the drug loading amount was 1040 mg/g). The formed MGO-TCA-FA@DOX has been developed to be an effective PCT nanoplatform with the advantages of multiple-targeted drug delivery, near-infrared light (NIR) and pH triggered drug release, and photothermal conversion efficiency. In vitro experiments showed that compared with other cancer cells and normal liver cells, MGO-TCA-FA@DOX could specifically target liver cancer cells and presented significant killing ability to liver cancer cells. More importantly, in vivo experiments indicated that PCT synergistic therapy (MGO-TCA-FA@DOX) revealed the best tumor inhibition (the tumor inhibition rate was about 85%) compared with chemotherapy and photothermal therapy alone. Thus, this study supplied a viable multiple-targeted PCT nano-agent for chemo-photothermal combination therapy of liver cancer.

光化学疗法（PCT）在肝细胞癌（HCC）治疗中显示出巨大的潜力，因此构建具有高光热转换效率和精确药物递送的智能PCT纳米剂具有重要意义。_{在此，通过在Fe 3} O ₄表面接枝三甲酰胆酸（TCA）和叶酸（FA），设计并构建了一种新型杂化纳米材料MGO-TCA-FA。改性氧化石墨烯（MGO）。盐酸多柔比星（DOX）作为模型药物可以通过氢键和π-π堆积有效负载在MGO-TCA-FA上（载药量为1040 mg/g）。形成的 MGO-TCA-FA@DOX 已被开发成为一种有效的 PCT 纳米平台，具有多靶向药物递送、近红外光 (NIR) 和 pH 触发药物释放以及光热转换效率等优点。体外实验表明，与其他癌细胞和正常肝细胞相比，MGO-TCA-FA@DOX可以特异性靶向肝癌细胞，对肝癌细胞具有显着的杀伤能力。更重要的是，体内实验表明，与单独的化学疗法和光热疗法相比，PCT协同疗法（MGO-TCA-FA@DOX）显示出最好的肿瘤抑制作用（肿瘤抑制率约为85％）。因此，本研究为肝癌的化学-光热联合治疗提供了一种可行的多靶点PCT纳米制剂。