A large fraction of metalloenzymes harbors multiple metal-centers that are electronically and/or functionally arranged within their proteinaceous environments. To explore the orchestration of inorganic and biochemical components and to develop bioinorganic catalysts and materials, we have described selected examples of artificial metalloproteins having multiple metallocofactors that were grouped depending on their initial protein scaffolds, the nature of introduced inorganic moieties, and the method used to propagate the number of metal ions within a protein. They demonstrated that diverse inorganic moieties can be selectively grafted and modulated in protein environments, providing a retrosynthetic bottom-up approach in the design of versatile and proficient biocatalysts and biomimetic model systems to explore fundamental questions in bioinorganic chemistry.

大部分金属酶含有多个金属中心，这些金属中心在其蛋白质环境中以电子和/或功能方式排列。为了探索无机和生化成分的协调并开发生物无机催化剂和材料，我们描述了具有多种金属因子的人造金属蛋白的选定示例，这些金属因子根据它们的初始蛋白质支架、引入的无机部分的性质以及用于制备的方法进行分组。传播蛋白质中金属离子的数量。他们证明了在蛋白质环境中可以选择性地接枝和调节不同的无机部分，