The consumption of vegetables grown in Pb-polluted soils causes serious threats to human health around the globe. In this study, we evaluated the Pb toxicity alleviation in water spinach grown of pot experiments in Pb-spiked soil treated with biochar and exogenous calcium. The results showed that both biochar and exogenous calcium alleviated Pb stress in water spinach, which was mainly manifested on its improved soil health and increased growth and decreased Pb uptake. Incorporation of 3% biochar significantly reduced CaCl₂ extracted Pb by 53.6% and decreased Pb accumulation in roots (67.1%) and shoots (80.8%). Our also findings indicated that Pb detoxification mechanism of biochar and exogenous calcium was totally different, while they can induce a synergistic impact on water spinach Pb stress alleviation. The combination of biochar and exogenous calcium in Pb-contaminated soil remediation may complement each other and reduce Pb entry into the human body through vegetables.

食用在铅污染土壤中种植的蔬菜对全球人类健康造成严重威胁。在这项研究中，我们评估了在用生物炭和外源钙处理的铅土壤中进行盆栽实验的空心菜中 Pb 毒性的缓解。结果表明，生物炭和外源钙均能缓解空心菜的铅胁迫，主要表现在改善土壤健康，促进生长，减少对铅的吸收。加入 3% 生物炭可显着降低 CaCl ₂提取 Pb 53.6% 并减少根 (67.1%) 和芽 (80.8%) 中的 Pb 积累。我们的研究结果还表明，生物炭和外源钙对铅的解毒机制完全不同，但它们对空心菜铅的缓解具有协同作用。生物炭与外源钙在铅污染土壤修复中的结合可以相得益彰，减少铅通过蔬菜进入人体。