Agriculture insecticides are used against insect pest species, but are able to change community structure in contaminated habitats, and also the genetic pool of exposed individuals. In fact, the latter effect is a relevant tool to in situ biomonitoring of pollutant contamination and impact, besides its practical economic and management concerns. This takes place because the emergence of individuals with resistance to insecticides is particularly frequent among insect pest species and usually enhances insecticide overuse and crop losses. Pest insects of global prominence such as whiteflies are a focus of attention due to problems with insecticide resistance and association with endosymbionts, as the case of the invasive putative species Bemisia tabaci MEAM1. The scenario is particularly complex in the Neotropics, where insecticide use is ubiquitous, but whose spatial scale of occurrence is usually neglected. Here we explored the spatial-dependence of both phenomena in MEAM1 whiteflies recording resistance to two widely used insecticides, lambda-cyhalothrin and spiromesifen, and endosymbiont co-occurrence. Resistance to both insecticides was frequent exhibiting low to moderate frequency of lambda-cyhalothrin resistance and moderate to high frequency of spiromesifen resistance. Among the prevailing whitefly endosymbionts, Wolbachia, Cardinium and Arsenophonus were markedly absent. In contrast, Hamiltonella and Rickettsia prevailed and their incidence was correlated. Furthermore, Rickettsia endosymbionts were particularly associated with lambda-cyhalothrin susceptibility. These traits were spatially dependent with significant variation taking place within an area of about 700 Km². Such findings reinforce the notion of endosymbiont-associated resistance to insecticides, and also of their local incidence allowing spatial mapping and locally-targeted mitigation.

农业杀虫剂用于防治害虫物种，但能够改变受污染栖息地的群落结构，以及暴露个体的遗传库。事实上，除了其实际的经济和管理问题外，后一种效应是污染物污染和影响的原位生物监测的相关工具。之所以发生这种情况，是因为在害虫物种中出现对杀虫剂具有抗性的个体尤为频繁，并且通常会加剧杀虫剂的过度使用和作物损失。由于杀虫剂抗药性和与内共生体相关的问题，粉虱等全球突出的害虫成为关注的焦点，例如侵入性假定物种烟粉虱MEAM1. 这种情况在新热带地区尤为复杂，在那里杀虫剂的使用无处不在，但其发生的空间尺度通常被忽视。在这里，我们探索了 MEAM1 粉虱记录对两种广泛使用的杀虫剂、λ-三氯氟氰菊酯和螺杀螨酯的抗性以及内共生菌共存现象的空间依赖性。对这两种杀虫剂的抗性经常表现出低至中等频率的 λ-三氯氟氰菊酯抗性和中至高频率的螺麦酯抗性。在流行的粉虱内共生体中，Wolbachia、Cardinium和Arsenophonus明显不存在。相反，汉密尔顿氏菌和立克次氏体占优势，并且它们的发病率相关。此外，立克次体内共生体与 λ-三氯氟氰菊酯敏感性特别相关。这些性状具有空间依赖性，在约 700 Km ²的区域内发生显着变异。这些发现强化了与内共生体相关的杀虫剂抗性的概念，以及它们的局部发生率允许空间映射和局部靶向缓解的概念。