The release of contaminants as herbicides, fungicides and insecticides into the environment has been listed as one of the six major contributors to the global decline of reptiles. Although reptiles may face severe risk from contaminants due to their ecology and physiology, they are currently less studied than other vertebrate groups. In the present work, we investigated if and how different types of field treatment (conventional and organic) affected the health status of Italian wall lizard (Podarcis siculus) individuals in central Italy. We chose a multi-biomarker approach that evaluated the biological responses of lizards to the treatment by means of AChE activity in the nervous system, biotransformation enzymes activities and oxidative stress in the liver, micronuclei frequency measured in the erythrocytes, and rate of intestinal parasitic infection. Our findings showed evidence of effects of treatment in conventional areas and between sexes with significant oxidative stress due to hydroxyl radicals, that caused DNA damage. No difference of intestinal parasite infections was found among treatments. Podarcis siculus seems to be a good bioindicator in ecotoxicological studies and potentially in risk assessment of pesticides, although further analyses in laboratory and in the field are needed to achieve more accurate quantification of specific pesticide effects in relation to known exposure history and to understand if other mechanisms were involved in the toxicity and detoxification process of pesticides for this species.

污染物以除草剂、杀菌剂和杀虫剂的形式释放到环境中，已被列为全球爬行动物数量下降的六大原因之一。尽管爬行动物由于其生态和生理学可能面临来自污染物的严重风险，但与其他脊椎动物群体相比，目前对它们的研究较少。在目前的工作中，我们调查了不同类型的野外处理（常规和有机）是否以及如何影响意大利壁蜥（Podarcis siculus）的健康状况) 意大利中部的个人。我们选择了一种多生物标志物方法，通过神经系统中的 AChE 活性、肝脏中的生物转化酶活性和氧化应激、红细胞中测量的微核频率以及肠道寄生虫感染率来评估蜥蜴对治疗的生物学反应. 我们的研究结果显示了在传统区域和性别之间治疗效果的证据，由于羟基自由基导致显着氧化应激，这会导致 DNA 损伤。各处理间肠道寄生虫感染无差异。波达卡斯 似乎是生态毒理学研究和农药风险评估中的一个很好的生物指标，尽管需要在实验室和现场进行进一步分析，以更准确地量化与已知接触历史相关的特定农药影响，并了解其他机制是否有效参与该物种农药的毒性和解毒过程。