The diamondback moth (Plutella xylostella L.), is an economic pest of cruciferous plants worldwide, which causes great economic loss to cruciferous plants production. However, the pest has developed resistance to insecticides. One of such insecticides is chlorantraniliprole. The study of the mechanisms underlying resistance is key for the effective management of resistance. In this study, a comparative proteomics approach was used to isolate and identify various proteins that differed between chlorantraniliprole-susceptible and -resistant strains of P. xylostella. Eleven proteins were significantly different and were successfully identified by MALDI-TOF-MS. Metabolism-related proteins accounted for the highest proportion among the eleven different proteins. The function of the PxGST2L protein was validated by RNAi. Knockdown of PxGST2L reduced the GST activity and increased the toxicity of chlorantraniliprole to the diamondback moth. The resistance ratio of diamondback moth to chlorantraniliprole was reduced from 1029 to 505. The results indicated that PxGST2L is partly responsible for chlorantraniliprole insecticide resistance in DBM. Our finding contributes to the understanding of the mechanism underlying resistance to chlorantraniliprole in the DBM, to develop effective resistance management tactics.

小菜蛾（Plutella xylostella L.）是世界范围内十字花科植物的经济害虫，给十字花科植物生产造成巨大的经济损失。然而，这种害虫已经对杀虫剂产生了抗药性。其中一种杀虫剂是氯虫苯甲酰胺。研究耐药性机制是有效管理耐药性的关键。在这项研究中，比较蛋白质组学方法用于分离和鉴定在小菜蛾的氯虫苯甲酰胺敏感和抗性菌株之间存在差异的各种蛋白质。11 种蛋白质显着不同，并通过 MALDI-TOF-MS 成功鉴定。代谢相关的蛋白质在十一种不同的蛋白质中所占的比例最高。的功能PxGST2L蛋白通过 RNAi 验证。敲除 PxGST2L 会降低 GST 活性并增加氯虫苯甲酰胺对小菜蛾的毒性。小菜蛾对氯虫苯甲酰胺的抗性比从1029降低到505。结果表明PxGST2L是导致DBM对氯虫苯甲酰胺杀虫剂抗药性的部分原因。我们的发现有助于了解 DBM 中对氯虫苯甲酰胺产生耐药性的潜在机制，从而制定有效的耐药性管理策略。