Folia Microbiologica ( IF 2.4 ) Pub Date : 2021-07-21 , DOI: 10.1007/s12223-021-00898-6 Petra Kašparová 1 , Martin Zmuda 1 , Eva Vaňková 1 , Olga Maťátková 1 , Jan Masák 1
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This study evaluated the effect of low-molecular weight chitosan on Staphylococcus epidermidis, a common colonizer of joint implants and other prosthetic devices. We have also attempted to elucidate its mechanism of action. Chitosan was found to be effective against both the planktonic and biofilm cells (MIC80 35–40 mg/L; MBIC80 40–150 mg/L), in contrast to the antibiotics erythromycin and tetracycline with no antibiofilm activity (MBIC80 not found). In combination, chitosan had an additive effect with antibiotics on suspension growth of S. epidermidis (FICi 0.7–1.0), and the combinatory action caused a complete inhibition of biofilm metabolic activity in some cases. In addition, chitosan caused rapid cellular damage and enhanced antihaemolytic activity of tetracycline in combination towards S. epidermidis biofilm cells. Chitosan efficiently inhibited S. epidermidis growth acting via cell membrane damage, yet the extent of antimicrobial and antibiofilm activities was quite strain-specific. It was proved to be a very efficient antimicrobial agent worth further examination as a potent candidate in pharmaceutical research. Apart from antimicrobial activity, it also acted as antivirulence enhancing agent which is a very promising strategy for alternative infectious diseases treatment.
Graphical abstract
中文翻译：
低分子量壳聚糖增强抗生素的抗菌作用并透化表皮葡萄球菌生物膜细胞的细胞质膜
本研究评估了低分子量壳聚糖对表皮葡萄球菌的影响，表皮葡萄球菌是关节植入物和其他假肢装置的常见定植菌。我们还试图阐明其作用机制。发现壳聚糖对浮游细胞和生物膜细胞均有效（MIC 80 35–40 mg/L；MBIC 80 40–150 mg/L），与没有抗生物膜活性的抗生素红霉素和四环素相反（未发现MBIC 80）。在组合中，壳聚糖与抗生素对表皮葡萄球菌的悬浮生长具有相加作用(FICi 0.7–1.0)，并且在某些情况下，组合作用导致生物膜代谢活动的完全抑制。此外，壳聚糖引起快速细胞损伤并增强四环素对表皮葡萄球菌生物膜细胞的抗溶血活性。壳聚糖通过细胞膜损伤有效抑制表皮葡萄球菌的生长，但抗菌和抗生物膜活性的程度是相当菌株特异性的。它被证明是一种非常有效的抗菌剂，值得进一步研究，作为药物研究中的有效候选物。除了抗菌活性外，它还可以作为抗毒力增强剂，这是替代传染病治疗的一种非常有前景的策略。
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