Objective

This study aimed to investigate the concentration of Wingless-type (Wnt)-inducible signaling pathway protein-1 (WISP1) in the serum, and its expression in the abdominal subcutaneous adipose tissue (SAT), and placenta of women with gestational diabetes mellitus (GDM).

Methods

A total of 69 patients with GDM and 71 pregnant women with normal glucose tolerance (NGT, control) were recruited. Carbohydrate metabolism, alanine aminotransferase (ALT), lipid profiles, thyroid function, interleukin-6 (IL-6), and WISP1 levels were assessed. Fasting sera were collected between 25 and 30 weeks of gestation. Tissues of placenta and abdominal SAT samples were obtained from 24 women who had undergone cesarean section and were divided into a GDM group and a control group. Reverse transcription polymerase chain reaction (RT-PCR) and western blot were used to detect the WISP1 expression.

Results

The serum WISP1 concentrations were higher in the GDM group than in the control group (p < 0.01) and positively associated with body mass index (BMI), fasting glucose, fasting insulin, HOMA-insulin resistance (HOMA-IR), and IL-6 levels. BMI, fasting glucose, and HOMA-IR independently and positively predicted WISP1 levels. Further, WISP1 mRNA and protein expression were higher in tissues from the placenta and abdominal SAT from the GDM group (p < 0.01).

Conclusions

WISP1 may be an important adipokine in modulating carbohydrate metabolism in women with GDM.

中文翻译：

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WISP1 在妊娠期糖尿病女性的母体血清、脂肪组织和胎盘中升高

客观的

本研究旨在探讨Wingless型（Wnt）诱导信号通路蛋白1（WISP1）在妊娠期糖尿病女性血清中的浓度及其在腹部皮下脂肪组织（SAT）和胎盘中的表达。 GDM）。

方法

共招募了 69 名 GDM 患者和 71 名糖耐量正常的孕妇（NGT，对照）。评估了碳水化合物代谢、丙氨酸氨基转移酶 (ALT)、脂质谱、甲状腺功能、白细胞介素 6 (IL-6) 和 WISP1 水平。在妊娠 25 至 30 周之间收集空腹血清。胎盘组织和腹部SAT样本取自24名剖宫产妇女，分为GDM组和对照组。逆转录聚合酶链反应 (RT-PCR) 和蛋白质印迹用于检测 WISP1 的表达。

结果

GDM组血清WISP1浓度高于对照组（p  < 0.01），并与体重指数（BMI）、空腹血糖、空腹胰岛素、HOMA-胰岛素抵抗（HOMA-IR）和IL- 6 级。BMI、空腹血糖和 HOMA-IR 独立且积极地预测 WISP1 水平。此外，来自 GDM 组的胎盘和腹部 SAT 组织中 WISP1 mRNA 和蛋白质表达较高（p  < 0.01）。

结论

WISP1 可能是调节 GDM 女性碳水化合物代谢的重要脂肪因子。