Introduction

Diabetic nephropathy causes chronic kidney disease and renal failure globally. We investigated the mRNA expression of resistin, TNF-α, and microRNA 124-3p (miR-124-3p) in peripheral blood mononuclear cells (PBMCs) of diabetic patients with and without nephropathy.

Methods

The mRNA expression of target genes in isolated PBMCs from whole blood was determined using real-time quantitative reverse transcription (RT) polymerase chain reaction (qRT-PCR). Demographic and clinical characteristics of study patients were also measured by standard methods.

Results

Relative expression of resistin and TNF-α was significantly increased (approximately 10-fold, and 1.5-fold respectively) in patients with nephropathy compared to that in patients without nephropathy (p < 0.001). However, the miR-124-3p expression was significantly decreased (approximately 10-fold) in patients with nephropathy in comparison to that in patients without nephropathy (p < 0.05). The expression of resistin was significantly associated with inflammatory markers such as creatinine, BUN, TNF-α, and miR-124-3p. The high expression of resistin was significantly associated with diabetic nephropathy (OR = 1.145; 95% C = 1.1–18.289, p = 0.024). The diagnostic performance of resistin, TNF-α, and miR-124-3p was significant based on ROC curve analysis (0.803, 95% CI: 0.723–0.926; 0.705, %95 CI: 0.559–0.851; and 0.707, 95% CI: 0.559–0.855, respectively). Thus, they can represent a diabetic biomarker for reflecting an advanced stage of the disease and inflammatory condition.

Conclusions

Our findings indicated that high expression of resistin in PBMCs is associated with diabetic nephropathy. Additionally, we suggested that the expression of resistin, TNF-α, and miR-124-3p in PBMCs of diabetic patients may be represented as predictive biomarkers for the diagnosis of nephropathy.

中文翻译：

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外周血单个核细胞中抵抗素、TNF-α 和 microRNA 124-3p 的表达与糖尿病肾病有关

介绍

糖尿病肾病在全球范围内导致慢性肾病和肾功能衰竭。我们研究了患有和不患有肾病的糖尿病患者的外周血单核细胞 (PBMC) 中抵抗素、TNF-α 和 microRNA 124-3p (miR-124-3p) 的 mRNA 表达。

方法

使用实时定量逆转录 (RT) 聚合酶链反应 (qRT-PCR) 测定从全血分离的 PBMC 中靶基因的 mRNA 表达。还通过标准方法测量了研究患者的人口统计学和临床​​特征。

结果

与非肾病患者相比，肾病患者抵抗素和 TNF-α 的相对表达显着增加（分别约为 10 倍和 1.5 倍）（p < 0.001）。然而，与非肾病患者相比，肾病患者的 miR-124-3p 表达显着降低（约 10 倍）（p < 0.05）。抵抗素的表达与炎症标志物如肌酐、BUN、TNF-α和miR-124-3p显着相关。抵抗素的高表达与糖尿病肾病显着相关（OR = 1.145；95% C = 1.1–18.289，p = 0.024）。根据 ROC 曲线分析，抵抗素、TNF-α 和 miR-124-3p 的诊断性能显着（0.803，95% CI：0.723-0.926；0.705，%95 CI：0.559-0.851；和 0.707，95% CI : 0.559–0.855, 分别）。因此，它们可以代表反映疾病晚期和炎症状况的糖尿病生物标志物。

结论

我们的研究结果表明，PBMC 中抵抗素的高表达与糖尿病肾病有关。此外，我们建议糖尿病患者 PBMC 中抵抗素、TNF-α 和 miR-124-3p 的表达可作为肾病诊断的预测生物标志物。