Objective. Local cooling of the brain as a therapeutic intervention is a promising alternative for patients with epilepsy who do not respond to medication. In vitro and in vivo studies have demonstrated the seizure-suppressing effect of local cooling in various animal models. In our work, focal brain cooling in a bicuculline induced epilepsy model in rats is demonstrated and evaluated using a multimodal micro-electrocorticography (microECoG) device. Approach. We designed and experimentally tested a novel polyimide-based sensor array capable of recording microECoG and temperature signals concurrently from the cortical surface of rats. The effect of cortical cooling after seizure onset was evaluated using 32 electrophysiological sites and eight temperature sensing elements covering the brain hemisphere, where injection of the epileptic drug was performed. The focal cooling of the cortex right above the injection site was accomplished using a miniaturized Peltier chip combined with a heat pipe to transfer heat. Control of cooling and collection of sensor data was provided by a custom designed Arduino based electronic board. We tested the experimental setup using an agar gel model in vitro, and then in vivo in Wistar rats. Main results. Spatial variation of temperature during the Peltier controlled cooling was evaluated through calibrated, on-chip platinum temperature sensors. We found that frequency of epileptic discharges was not substantially reduced by cooling the cortical surface to 30 C, but was suppressed efficiently at temperature values around 20 C. The multimodal array revealed that seizure-like ictal events far from the focus and not exposed to high drop in temperature can be also inhibited at an extent like the directly cooled area. Significance. Our results imply that not only the absolute drop in temperature determines the efficacy of seizure suppression, and distant cortical areas not directly cooled can be influenced.

客观的。对于对药物无反应的癫痫患者，大脑的局部冷却作为治疗干预是一种很有前景的替代方法。体外和体内研究已经证明了局部冷却在各种动物模型中的癫痫抑制作用。在我们的工作中，使用多模式微皮层电图 (microECoG) 设备演示和评估了荷包牡丹碱诱导的大鼠癫痫模型中的局灶性脑冷却。方法。我们设计并实验测试了一种新型的基于聚酰亚胺的传感器阵列，能够同时记录来自大鼠皮层表面的 microECoG 和温度信号。使用 32 个电生理位点和覆盖大脑半球的 8 个温度传感元件评估癫痫发作后皮质冷却的效果，在那里进行癫痫药物注射。注射部位正上方皮层的局部冷却是使用微型珀耳帖芯片与热管相结合来传递热量来实现的。冷却控制和传感器数据收集由定制设计的基于 Arduino 的电子板提供。我们在体外使用琼脂凝胶模型测试了实验设置，然后在 Wistar 大鼠中进行了体内测试。主要结果。通过校准的片上铂温度传感器评估 Peltier 控制冷却过程中的温度空间变化。我们发现通过将皮质表面冷却至 30 C 并没有显着降低癫痫放电的频率，但在 20 C 左右的温度值下被有效抑制。多模式阵列显示癫痫样发作事件远离焦点且未暴露于高也可以像直接冷却区域那样抑制温度下降。意义。我们的结果意味着，不仅温度的绝对下降决定了癫痫抑制的功效，而且未直接冷却的远处皮质区域也会受到影响。