Background:

Glioma is the central nervous system tumor with the highest incidence rate and the molecular detection of gliomas has been the focus of research. This study aimed to investigate the guiding effect of cluster of differentiation 276 (CD276) expression on the clinical prognosis of glioma.

Methods:

The TCGA and CGGA databases were used to study whether CD 276 can be used as an independent prognostic factor for gliomas. Immunohistochemistry was used to detect the expression of CD276, isocitrate dehydrogenase-1 (IDH1), matrix metallopeptidase 9 (MMP9), p53, and Ki-67, and 1p/19q co-deletion was detected by fluorescence in situ hybridization (FISH). The effects of CD276 RNA interference (RNAi) on cell invasion, cell cycle and the expression of β-catenin, tumor necrosis factor receptor 1 (TNFR1), and MMP9 were observed. Furthermore, the biological effects of CD276 gene knockout on intracranial transplanted tumors in nude mice were studied.

Results:

CD276 expression was positively correlated with the extracellular matrix, collagen decomposition, and cell adhesion molecules. Immunohistochemistry and FISH showed that CD276 expression positively correlated with the glioma grade, p53 mutation, Ki-67 proliferation, and MMP9 expression; however, it negatively correlated with IDH1 mutation, 1p/19q co-deletion, and the survival rate. CD276 RNAi in U87 cells inhibited cell proliferation, migration, and invasion, but had no effect on the cell cycle. CD276 inhibited the expression of β-catenin, TNFR1, and MMP9 in U87 cells at the mRNA and protein levels. In vivo experiments showed that the tumor formation and invasion of the CD276 small interfering RNA glioma cell line in nude mice were reduced and the survival time was prolonged.

Conclusions:

The present study demonstrated that high expression of CD276 in gliomas indicates a poor prognosis.

中文翻译：

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分化簇276的高表达表明胶质瘤预后不良

背景：

胶质瘤是发病率最高的中枢神经系统肿瘤，胶质瘤的分子检测一直是研究的重点。本研究旨在探讨分化簇276（CD276）表达对胶质瘤临床预后的指导作用。

方法：

TCGA 和 CGGA 数据库用于研究 CD 276 是否可以用作神经胶质瘤的独立预后因素。免疫组化检测CD276、异柠檬酸脱氢酶1（IDH1）、基质金属肽酶9（MMP9）、p53、Ki-67的表达，荧光原位杂交（FISH）检测1p/19q共缺失。观察CD276 RNA干扰（RNAi）对细胞侵袭、细胞周期及β-catenin、肿瘤坏死因子受体1（TNFR1）和MMP9表达的影响。此外，还研究了CD276基因敲除对裸鼠颅内移植肿瘤的生物学效应。

结果：

CD276 表达与细胞外基质、胶原分解和细胞粘附分子呈正相关。免疫组化和FISH显示CD276表达与胶质瘤分级、p53突变、Ki-67增殖、MMP9表达呈正相关；然而，它与 IDH1 突变、1p/19q 共缺失和存活率呈负相关。U87细胞中的CD276 RNAi抑制细胞增殖、迁移和侵袭，但对细胞周期没有影响。CD276 在 mRNA 和蛋白质水平抑制 U87 细胞中 β-catenin、TNFR1 和 MMP9 的表达。体内实验表明，CD276小干扰RNA胶质瘤细胞株在裸鼠体内的肿瘤形成和侵袭减少，存活时间延长。

结论：

本研究表明，胶质瘤中 CD276 的高表达表明预后不良。