Curcumin is an active ingredient isolated from the rhizomes of Curcuma longa Linn with remarkably non-toxic bioavailability. This is an in vitro study. In this study, we explored the effects of curcumin on the proliferation, migration and neurogenesis of neural stem cells (NSCs). Primary NSCs were isolated from embryonic day 14 rats and then treated with curcumin and/or stromal derived factor-1 (SDF-1). NSCs showed an SDF-1-dependent proliferation and migration. Further results showed that curcumin and SDF-1 both promoted NSCs proliferation, migration and the formation of neurospheres. In addition, Curcumin up-regulated the expression of SDF-1 and promoted the formation of SDF-1/CXCR4 complex in NSCs. The western blot results showed that the phosphorylation levels of ERK, JNK, MAPK, NF-kB and Akt were up-regulated by curcumin. In contrast, the administration of CXCR4 inhibitor AMD3100 could offset the effect of curcumin. These results suggested that curcumin promoted the NSCs proliferation, migration and formation of neurospheres via SDF-1/CXCR4 in NSCs.

中文翻译：

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姜黄素通过激活SDF-1/CXCR4轴促进神经干细胞的增殖、侵袭和神经球的形成

姜黄素是一种从姜黄根茎中分离出来的活性成分，具有非常无毒的生物利用度。这是一项体外研究。在这项研究中，我们探讨了姜黄素对神经干细胞 (NSC) 增殖、迁移和神经发生的影响。从胚胎第 14 天的大鼠中分离出原代 NSC，然后用姜黄素和/或基质衍生因子-1 (SDF-1) 处理。NSCs 表现出 SDF-1 依赖性增殖和迁移。进一步的结果表明姜黄素和 SDF-1 均能促进 NSCs 的增殖、迁移和神经球的形成。此外，姜黄素上调 SDF-1 的表达并促进 NSCs 中 SDF-1/CXCR4 复合物的形成。Western blot结果显示姜黄素可上调ERK、JNK、MAPK、NF-kB和Akt的磷酸化水平。相比之下，CXCR4抑制剂AMD3100的给药可以抵消姜黄素的作用。这些结果表明姜黄素通过 NSCs 中的 SDF-1/CXCR4 促进 NSCs 的增殖、迁移和神经球的形成。