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Hsa_circ_0001017 inhibits proliferation and metastasis via regulating the let-7g-3p/NDST3 axis in glioma
Folia Neuropathologica ( IF 1.5 ) Pub Date : 2021-06-30 , DOI: 10.5114/fn.2021.107115 Tao Li 1 , Yunlong Li 2 , Wei Zhang 3 , Jing Zhang 4
Folia Neuropathologica ( IF 1.5 ) Pub Date : 2021-06-30 , DOI: 10.5114/fn.2021.107115 Tao Li 1 , Yunlong Li 2 , Wei Zhang 3 , Jing Zhang 4
Affiliation
Introduction
Glioma is one of the common types of intracranial malignancies, which seriously threatens human health. Circular RNAs (circRNAs) play critical roles in various tumours. This study aims to observe the functions and mechanism of circ_0001017 in glioma progression.
Material and methods
Hsa_circ_0001017 expression was analysed in glioma tissue and cells using qRT-PCR assay. Flow cytometer, Cell Counting Kit-8 (CCK-8), colony formation, wound healing, and Transwell were used to analyse glioma cell apoptosis, viability, colony-forming ability, migration, and invasion. The expression of N-deacetylase and N-sulfotransferase 3 (NDST3) and epithelial-mesenchymal transition (EMT)-related proteins was measured using western blot analysis. Luciferase reporter and RNA immunoprecipitation (RIP) assay were performed to determine the target relationship.
Results
In glioma tissues and cells, hsa_circ_0001017 expression was decreased. The overexpression of hsa_circ_0001017 inhibited glioma cell proliferation, EMT, migration, and invasion and promoted glioma cell apoptosis, while the knockdown of hsa_circ_0001017 caused the opposite results. Mechanistically, hsa_circ_0001017 sponged hsa-let-7g-3p and NDST3 was the target gene of hsa-let-7g-3p. Moreover, the tumour-suppressive role of circ_0001017 was associated with hsa-let-7g-3p and NDST3.
Conclusions
Hsa_circ_0001017 suppressed the growth and metastasis and increased cell apoptosis of glioma, and this effect was associated with hsa-let-7g-3p/NDST3 axis.
中文翻译：
Hsa_circ_0001017通过调节let-7g-3p/NDST3轴抑制胶质瘤的增殖和转移
简介
胶质瘤是颅内恶性肿瘤的常见类型之一，严重威胁人类健康。环状 RNA (circRNA) 在各种肿瘤中发挥关键作用。本研究旨在观察circ_0001017在胶质瘤进展中的作用和机制。
材料与方法
使用 qRT-PCR 测定法分析胶质瘤组织和细胞中的 Hsa_circ_0001017 表达。流式细胞仪、Cell Counting Kit-8 (CCK-8)、集落形成、伤口愈合和 Transwell 用于分析胶质瘤细胞凋亡、活力、集落形成能力、迁移和侵袭。使用蛋白质印迹分析测量 N-脱乙酰酶和 N-磺基转移酶 3 (NDST3) 和上皮间质转化 (EMT) 相关蛋白的表达。进行荧光素酶报告基因和 RNA 免疫沉淀 (RIP) 测定以确定目标关系。
结果
在胶质瘤组织和细胞中，hsa_circ_0001017 表达降低。hsa_circ_0001017的过表达抑制胶质瘤细胞增殖、EMT、迁移和侵袭，促进胶质瘤细胞凋亡，而hsa_circ_0001017的敲低则导致相反的结果。机制上，hsa_circ_0001017海绵化了hsa-let-7g-3p，NDST3是hsa-let-7g-3p的靶基因。此外，circ_0001017 的肿瘤抑制作用与 hsa-let-7g-3p 和 NDST3 相关。
结论
Hsa_circ_0001017抑制胶质瘤的生长和转移，增加细胞凋亡，该作用与hsa-let-7g-3p/NDST3轴相关。
更新日期：2021-07-21
Glioma is one of the common types of intracranial malignancies, which seriously threatens human health. Circular RNAs (circRNAs) play critical roles in various tumours. This study aims to observe the functions and mechanism of circ_0001017 in glioma progression.
Material and methods
Hsa_circ_0001017 expression was analysed in glioma tissue and cells using qRT-PCR assay. Flow cytometer, Cell Counting Kit-8 (CCK-8), colony formation, wound healing, and Transwell were used to analyse glioma cell apoptosis, viability, colony-forming ability, migration, and invasion. The expression of N-deacetylase and N-sulfotransferase 3 (NDST3) and epithelial-mesenchymal transition (EMT)-related proteins was measured using western blot analysis. Luciferase reporter and RNA immunoprecipitation (RIP) assay were performed to determine the target relationship.
Results
In glioma tissues and cells, hsa_circ_0001017 expression was decreased. The overexpression of hsa_circ_0001017 inhibited glioma cell proliferation, EMT, migration, and invasion and promoted glioma cell apoptosis, while the knockdown of hsa_circ_0001017 caused the opposite results. Mechanistically, hsa_circ_0001017 sponged hsa-let-7g-3p and NDST3 was the target gene of hsa-let-7g-3p. Moreover, the tumour-suppressive role of circ_0001017 was associated with hsa-let-7g-3p and NDST3.
Conclusions
Hsa_circ_0001017 suppressed the growth and metastasis and increased cell apoptosis of glioma, and this effect was associated with hsa-let-7g-3p/NDST3 axis.
中文翻译：
Hsa_circ_0001017通过调节let-7g-3p/NDST3轴抑制胶质瘤的增殖和转移
简介
胶质瘤是颅内恶性肿瘤的常见类型之一，严重威胁人类健康。环状 RNA (circRNA) 在各种肿瘤中发挥关键作用。本研究旨在观察circ_0001017在胶质瘤进展中的作用和机制。
材料与方法
使用 qRT-PCR 测定法分析胶质瘤组织和细胞中的 Hsa_circ_0001017 表达。流式细胞仪、Cell Counting Kit-8 (CCK-8)、集落形成、伤口愈合和 Transwell 用于分析胶质瘤细胞凋亡、活力、集落形成能力、迁移和侵袭。使用蛋白质印迹分析测量 N-脱乙酰酶和 N-磺基转移酶 3 (NDST3) 和上皮间质转化 (EMT) 相关蛋白的表达。进行荧光素酶报告基因和 RNA 免疫沉淀 (RIP) 测定以确定目标关系。
结果
在胶质瘤组织和细胞中，hsa_circ_0001017 表达降低。hsa_circ_0001017的过表达抑制胶质瘤细胞增殖、EMT、迁移和侵袭，促进胶质瘤细胞凋亡，而hsa_circ_0001017的敲低则导致相反的结果。机制上，hsa_circ_0001017海绵化了hsa-let-7g-3p，NDST3是hsa-let-7g-3p的靶基因。此外，circ_0001017 的肿瘤抑制作用与 hsa-let-7g-3p 和 NDST3 相关。
结论
Hsa_circ_0001017抑制胶质瘤的生长和转移，增加细胞凋亡，该作用与hsa-let-7g-3p/NDST3轴相关。
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