Introduction
Glioma is the most common primary brain tumour in adults. Numerous studies have shown that neuregulins (NRGs) may be involved in the formation of glioma. Although NRG1 has been extensively studied in glioma, the functions of NRG2 in glioma development remain elusive.

Material and methods
In the present study, we investigated the expression of NRG2 in different grades of human glioma samples, and analysed the functional effects of NRG2 in glioma cells mainly using wound healing assay and transmigration assay.

Results
We found that NRG2 was differentially expressed in different grades of human glioma/glioblastoma tissues. The data from wound healing assays demonstrated that NRG2 can differentially promote the migration of SHG44 human glioma, and U251 and U-87 MG human glioblastoma cells at different time points. The results of cell transmigration assays showed that, compared with the vehicle control, the number of cells that migrated to the underside of the insert was increased significantly for all the 3 cell lines treated with 5 nM of NRG2 for 12 hours.

Conclusions
In conclusion, our results demonstrated that NRG2 is expressed in gliomas to varying extents, and it may play roles in the migration of glioma cells in vitro. These data suggest that treatment targeting NRG2 signalling may partly reverse the migration-based metastasis of glioma cells.



中文翻译：

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Neuregulin 2 (NRG2) 在胶质瘤中表达并促进人胶质瘤细胞的迁移

简介
胶质瘤是成人最常见的原发性脑肿瘤。大量研究表明，神经调节蛋白 (NRG) 可能参与神经胶质瘤的形成。尽管 NRG1 已在神经胶质瘤中进行了广泛研究，但 NRG2 在神经胶质瘤发展中的功能仍然难以捉摸。

材料与方法
本研究主要通过伤口愈合试验和迁移试验分析了NRG2在不同级别人脑胶质瘤样本中的表达，并分析了NRG2在胶质瘤细胞中的功能作用。

结果
我们发现 NRG2 在不同等级的人胶质瘤/胶质母细胞瘤组织中存在差异表达。来自伤口愈合试验的数据表明，NRG2 可以在不同时间点差异促进 SHG44 人胶质瘤、U251 和 U-87 MG 人胶质母细胞瘤细胞的迁移。细胞迁移测定的结果表明，与载体对照相比，迁移到插入物下侧的细胞数量对于用 5 nM NRG2 处理 12 小时的所有 3 种细胞系都显着增加。

结论
总之，我们的研究结果表明，NRG2 在胶质瘤中不同程度地表达，它可能在胶质瘤细胞的体外迁移中发挥作用。这些数据表明，靶向 NRG2 信号的治疗可能部分逆转胶质瘤细胞基于迁移的转移。