British Poultry Science ( IF 1.6 ) Pub Date : 2021-08-13 , DOI: 10.1080/00071668.2021.1958298 S Uribe-Diaz 1, 2 , N Nazeer 2 , J Jaime 3 , D S Vargas-Bermúdez 3 , A Yitbarek 4 , M Ahmed 2 , J C Rodríguez-Lecompte 1
ABSTRACT
1. This study evaluated the effect of folic acid (FA) supplementation on the proinflammatory and antiviral molecular pathways of B-lymphocytes infected with a modified live IBDV (ST-12) mild vaccine strain during a timed post-infection analysis.
2. A chicken B-lymphocytes (DT-40) cell line was cultured in triplicate at a concentration of 5 × 105 cells per well in 24-well plates; and was divided into three groups: 1: No virus, FA; 2: Virus, no FA; 3: Virus + FA at a concentration of 3.96 mM. The experiment was repeated three times.
3. Cells in groups 2 and 3 were infected with a modified live IBDV (ST-12) mild vaccine strain at one multiplicity of infection (MOI: 1). After 1 hour of virus adsorption, samples were collected at 0, 3, 6, 12, 24 and 36 hours post-infection (hpi).
4. The modified live IBDV (ST-12) mild vaccine strain triggered a B-lymphocyte specific immune response associated with the upregulation of genes involved in virus recognition (Igß), virus sensing (TLR-2, TLR-3, TLR-4 and MDA5), signal transduction and regulation (TRIF, MyD88 and IRF7), and the antiviral effector molecules (IFN-α, OAS, PKR, and viperin).
5. FA supplementation modulated IBDV replication and regulated the proinflammatory and antiviral downstream molecular pathways.
6. In conclusion, the low virulent pathotype serotype I modified live IBDV (ST-12) mild vaccine strain was able to trigger and mount an immune response in chicken B-lymphocytes without affecting B-cell viability. FA supplementation modulated B lymphocytes response and improved their innate immune proinflammatory and antiviral response molecular pathways.
中文翻译:
叶酸增强感染轻度传染性法氏囊病病毒的鸡 B 淋巴细胞的促炎和抗病毒分子途径
摘要
1. 本研究在感染后的定时分析中评估了补充叶酸 (FA) 对感染改良活 IBDV (ST-12) 温和疫苗株的 B 淋巴细胞的促炎和抗病毒分子途径的影响。
2.将鸡B淋巴细胞(DT-40)细胞系以每孔5×10 5 个细胞的浓度在24孔板中一式三份培养;并分为三组:1:无病毒,FA;2:病毒,无FA;3:浓度为 3.96 mM 的病毒 + FA。实验重复了 3 次。
3. 第 2 组和第 3 组的细胞以一种感染复数 (MOI: 1) 用改良的活 IBDV (ST-12) 温和疫苗株感染。病毒吸附 1 小时后,在感染后 0、3、6、12、24 和 36 小时 (hpi) 收集样本。
4. 改良的活 IBDV (ST-12) 温和疫苗株引发了与病毒识别 (Igß)、病毒感应 (TLR-2、TLR-3、TLR-4) 相关基因上调相关的 B 淋巴细胞特异性免疫反应和 MDA5)、信号转导和调节(TRIF、MyD88 和 IRF7)以及抗病毒效应分子(IFN-α、OAS、PKR 和毒蛇素)。
5. FA 补充剂调节 IBDV 复制并调节促炎和抗病毒下游分子途径。
6. 总之,低毒力致病型血清型 I 修饰的活 IBDV (ST-12) 温和疫苗株能够在鸡 B 淋巴细胞中触发和产生免疫反应,而不会影响 B 细胞活力。FA 补充剂调节 B 淋巴细胞反应并改善其先天免疫促炎和抗病毒反应分子途径。