Abstract

Essential oils from the leaves of Vernonia patula (Dryand.) Merr. and Grangea maderaspatana (L.) Poir. collected from Pu Hoat Nature Reserve, Nghe An Province were studied for their chemical constituents and antimicrobial activity. The main constituents of V. patula were the sesquiterpenes namely β-caryophyllene (28.5 %), caryophyllene oxide (16.6 %), α-copaene (9.0 %), and α-humulene (7.1 %). On the other hand, a mixture of monoterpenes and sesquiterpenes hydrocarbons represented by myrecene (27.7 %), α-humulene (19.7 %), and germacrene D (15.8 %) were the main compounds of essential oils from the leaves of G. maderaspatana. Both essential oils displayed antimicrobial activity against the tested Gram-positive and Gram-negative bacteria with the minimum inhibitory concentrations (MIC) less than 50.0 μg/mL (8.67-24.56 μg/mL). The leaf essential oil of V. patula was the most potent towards Enterococcus faecalis (ATCC 299212) with MIC value of 8.67 μg/mL. The essential oils also showed anti-candidal properties with MIC values of 15.99 μg/mL (V. patula) and 65.67 μg/mL (G. maderaspatana). The chemical components and antimicrobial activity of the V. patula leaf essential oil was reported for the first time.

摘要

来自斑鸠菊(Dryand.) Merr叶子的精油。和Grangea maderaspatana (L.) Poir。研究了从义安省 Pu Hoat 自然保护区收集的化学成分和抗菌活性。V. patula的主要成分是倍半萜烯，即 β-石竹烯 (28.5%)、石竹烯氧化物 (16.6%)、α-可帕烯 (9.0%) 和 α-葎草烯 (7.1%)。另一方面，以月桂烯 (27.7%)、α-葎草烯 (19.7%) 和锗烯 D (15.8%) 为代表的单萜和倍半萜烃的混合物是马德拉斯帕塔 ( G. maderaspatana)叶子精油的主要化合物。两种精油都对测试的革兰氏阳性菌和革兰氏阴性菌显示出抗菌活性，最低抑菌浓度 (MIC) 低于 50.0 μg/mL (8.67-24.56 μg/mL)。V. patula的叶精油对粪肠球菌(ATCC 299212) 的作用最强，MIC 值为 8.67 μg/mL。精油还显示出抗念珠菌特性，MIC 值为 15.99 μg/mL ( V. patula ) 和 65.67 μg/mL ( G. maderaspatana )。首次报道了V. patula叶精油的化学成分和抗菌活性。