Abstract

In the current study, the chemical constituents of leaf essential oil from Cananga odorata (Lam.) Hook. F. & Thomson were identified by GC/MS analysis. A total of 25 components (91.1 %) in the essential oil were identified. The major compounds present in the oil were trans-caryophyllene (19.7 %), ocimene (13.2 %), E, E-α-farnesene (10.3 %), phenylmethyl ester (5.6 %), farnesyl acetone (5.1 %), t-muurolol (4.2 %), farnesol (3.7 %), β-elemene (3.1 %), α-cadinol (3.0 %), copaene (2.9 %), benzyl benzoate (2.3 %), trans-farnesol (2.1 %). The essential oil is rich in sesquiterpenoids including sesquiterpenes hydrocarbon (42.3 %), monoterpene hydrocarbons (18.1 %), and in addition to that oxygenated compound representing (30.7 %). The Antioxidant efficiency of essential oil of C. odorata leaves was performed by DPPH radical method together with BHA as standard. The essential oil showed concentration-dependent antiradical activity with EC₅₀ value of 19.5±1.25 μg/mL, when compared with standard BHA which showed EC₅₀ value of 1.10±0.55 μg/mL. The in vitro anticancer potential of essential oil was evaluated by using MOLT-3 cell line by MTT assay. The C. odorata essential oil exhibited significant anticancer activity with IC₅₀ value of 44.22 μg/mL. From the results, C. odorata essential oil has significant antioxidant and anticancer properties which can be utilised as a natural supplement as antioxidant and anticancer agents in pharmaceutical and food industries since it is available throughout the year.

摘要

在目前的研究中，来自Cananga odorata (Lam.) Hook的叶子精油的化学成分。F. & Thomson 通过 GC/MS 分析进行鉴定。鉴定出精油中总共 25 种成分 (91.1%)。油中存在的主要化合物是反式石竹烯 (19.7 %)、罗勒烯 (13.2 %)、E, E-α-法呢烯 (10.3 %)、苯甲酯 (5.6 %)、法呢基丙酮 (5.1 %)、t- muurolol (4.2 %)、法尼醇 (3.7 %)、β-榄香烯 (3.1 %)、α-卡地诺 (3.0 %)、可巴烯 (2.9 %)、苯甲酸苄酯 (2.3 %)、反式-法呢醇 (2.1%)。精油富含倍半萜类化合物，包括倍半萜烃 (42.3 %)、单萜烃 (18.1 %)，此外还含有含氧化合物 (30.7 %)。C. odorata叶精油的抗氧化效率是通过 DPPH 自由基法以 BHA 为标准进行的。与显示出1.10±0.55 μg/mL 的EC ₅₀值的标准 BHA 相比，精油显示出浓度依赖性的抗自由基活性，EC ₅₀值为 19.5±1.25 μg/mL。在体外精油的抗癌潜力通过使用MOLT-3细胞系通过MTT测定法来评估。的飞机草C.精油表现出显著抗癌活性与IC₅₀值为 44.22 μg/mL。结果表明，香樟精油具有显着的抗氧化和抗癌特性，可作为天然补充剂用作制药和食品行业的抗氧化剂和抗癌剂，因为它全年可用。