Upland cotton is the most widely planted for natural fiber around the world, and either lint percentage (LP) or fiber length (FL) is the crucial component tremendously affecting cotton yield and fiber quality, respectively. In this study, two lines MBZ70-053 and MBZ70-236 derived from G. hirsutum CCRI70 recombinant inbred line (RIL) population presenting different phenotypes in LP and FL traits were chosen to conduct RNA sequencing on ovule and fiber samples, aiming at exploring the differences of molecular and genetic mechanisms during cotton fiber initiation and elongation stages. As a result, 249/128, 369/206, 4296/1198 and 3547/2129 up-/down- regulated differentially expressed genes (DGEs) in L2 were obtained at −3, 0, 5 and 10 days post-anthesis (DPA), respectively. Seven gene expression profiles were discriminated using Short Time-series Expression Miner (STEM) analysis; seven modules and hub genes were identified using weighted gene co-expression network analysis. The DEGs were mainly enriched into energetic metabolism and accumulating as well as auxin signaling pathway in initiation and elongation stages, respectively. Meanwhile, 29 hub genes were identified as 14-3-3ω, TBL35, GhACS, PME3, GAMMA-TIP, PUM-7, etc., where the DEGs and hub genes revealed the genetic and molecular mechanisms and differences during cotton fiber development.

中文翻译：

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纤维起始和伸长的共表达网络和比较转录组分析揭示了陆地棉 CCRI70 RIL 种群两个品系的遗传差异

陆地棉是世界上种植最广泛的天然纤维，皮棉百分比 (LP) 或纤维长度 (FL) 是分别极大影响棉花产量和纤维质量的关键因素。本研究选择来自陆地棉 CCRI70 重组自交系 (RIL) 群体的两个品系 MBZ70-053 和 MBZ70-236，在 LP 和 FL 性状中表现出不同的表型，对胚珠和纤维样品进行 RNA 测序，旨在探索棉花纤维起始和伸长阶段分子和遗传机制的差异。结果，在开花后 -3、0、5 和 10 天（DPA ）， 分别。使用短时间序列表达挖掘器 (STEM) 分析区分了七个基因表达谱；使用加权基因共表达网络分析确定了七个模块和中心基因。DEGs主要分别在起始和延伸阶段富集到能量代谢和积累以及生长素信号通路中。同时鉴定出14-3-3ω、TBL35、GhACS、PME3、GAMMA-TIP、PUM-7等29个hub基因，其中DEGs和hub基因揭示了棉花纤维发育过程中的遗传和分子机制及差异。分别。同时鉴定出14-3-3ω、TBL35、GhACS、PME3、GAMMA-TIP、PUM-7等29个hub基因，其中DEGs和hub基因揭示了棉花纤维发育过程中的遗传和分子机制及差异。分别。同时鉴定出14-3-3ω、TBL35、GhACS、PME3、GAMMA-TIP、PUM-7等29个hub基因，其中DEGs和hub基因揭示了棉花纤维发育过程中的遗传和分子机制及差异。